Chen Dong, Wang Qi, Ma Zhi-Wei, Chen Fa-Ming, Chen Yue, Xie Guang-Yuan, Wang Qin-Tao, Wu Zhi-Fen
Department of Periodontology and Oral Medicine, School of Stomatology, China.
J Clin Periodontol. 2007 May;34(5):384-9. doi: 10.1111/j.1600-051X.2007.01071.x.
Aggressive periodontitis (AgP) has a genetic basis. It has been reported that the functional gene polymorphisms of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitor of metalloproteinase-2 (TIMP-2) alter their expressions in transcriptional level and they are involved in the tissue destruction of periodontitis. The study was carried out to analyse the association of functional polymorphisms in MMP-2, MMP-9 and TIMP-2 with generalized AgP (G-AgP) in a Chinese population.
The study population consisted of 79 Chinese patients with G-AgP and 128 healthy controls. DNA was obtained from oral mucosa swab samples. MMP-2 genotypes were determined by PCR-based denaturing high-performance liquid chromatography analysis while MMP-9 and TIMP-2 genotypes were identified by a PCR-based restriction fragment length polymorphism. Chi2 test after Yates' correction was used to investigate the possible association of the genotypes with the G-AgP.
Although gene polymorphisms for MMP-2 and MMP-9 did not show any association with the G-AgP, the analysis of the TIMP-2 -418G to C gene polymorphism revealed significant differences between the patients and controls. Compared with controls, a significant increasing trend of TIMP-2 -418C carrier in the G-AgP patients occurred (p=0.013).
It is suggested that the TIMP2 -418G to C gene polymorphism is associated with G-AgP in the Chinese subjects.
侵袭性牙周炎（AgP）具有遗传基础。据报道，基质金属蛋白酶（MMP）-2、MMP-9和金属蛋白酶组织抑制剂-2（TIMP-2）的功能基因多态性会在转录水平改变它们的表达，并且它们参与了牙周炎的组织破坏过程。本研究旨在分析中国人群中MMP-2、MMP-9和TIMP-2功能多态性与广泛型AgP（G-AgP）的相关性。
研究人群包括79名中国G-AgP患者和128名健康对照者。从口腔黏膜拭子样本中提取DNA。MMP- 的基因型通过基于聚合酶链反应（PCR）的变性高效液相色谱分析来确定，而MMP-9和TIMP-2的基因型通过基于PCR的限制性片段长度多态性来鉴定。采用Yates校正后的卡方检验来研究基因型与G-AgP的可能相关性。
尽管MMP-2和MMP-9的基因多态性与G-AgP没有显示出任何相关性，但对TIMP-2 -418G→C基因多态性的分析显示患者和对照之间存在显著差异。与对照组相比，G-AgP患者中TIMP-2 -418C携带者有显著增加的趋势（p = 0.013）。
提示TIMP2 -418G→C基因多态性与中国人群中的G-AgP相关。 2
