Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Ghent, Belgium.
Microb Biotechnol. 2008 Nov;1(6):487-96. doi: 10.1111/j.1751-7915.2008.00049.x.
In many microbial bioreactors, high shear rates result in strong attachment of microbes and dense biofilms. In this study, high shear rates were applied to enrich an anodophilic microbial consortium in a microbial fuel cell (MFC). Enrichment at a shear rate of about 120 s(-1) resulted in the production of a current and power output two to three times higher than those in the case of low shear rates (around 0.3 s(-1)). Biomass and biofilm analyses showed that the anodic biofilm from the MFC enriched under high shear rate conditions, in comparison with that under low shear rate conditions, had a doubled average thickness and the biomass density increased with a factor 5. The microbial community of the former, as analysed by DGGE, was significantly different from that of the latter. The results showed that enrichment by applying high shear rates in an MFC can result in a specific electrochemically active biofilm that is thicker and denser and attaches better, and hence has a better performance.
在许多微生物生物反应器中，高剪切速率会导致微生物强烈附着和形成密集的生物膜。在这项研究中，高剪切速率被应用于微生物燃料电池 (MFC) 中富集好氧微生物群落。在约 120 s(-1) 的剪切速率下进行富集，会导致电流和功率输出比低剪切速率（约 0.3 s(-1)) 下高出两到三倍。生物量和生物膜分析表明，与低剪切速率条件下相比，在高剪切速率条件下富集的阳极生物膜的平均厚度增加了一倍，生物量密度增加了 5 倍。通过 DGGE 分析，前者的微生物群落与后者有显著差异。结果表明，在 MFC 中施加高剪切速率进行富集可以得到一种特殊的电化学活性生物膜，该生物膜更厚、更密集、附着更好，因此具有更好的性能。
