Biffinger Justin C, Pietron Jeremy, Ray Ricky, Little Brenda, Ringeisen Bradley R
Chemistry Division, Naval Research Laboratory, 4555 Overlook Avenue, SW, Washington, DC 20375, USA.
Biosens Bioelectron. 2007 Mar 15;22(8):1672-9. doi: 10.1016/j.bios.2006.07.027. Epub 2006 Aug 30.
A miniature-microbial fuel cell (mini-MFC, chamber volume: 1.2 mL) was used to monitor biofilm development from a pure culture of Shewanella oneidensis DSP10 on graphite felt (GF) under minimal nutrient conditions. ESEM evidence of biofilm formation on GF is supported by substantial power density (per device cross-section) from the mini-MFC when using an acellular minimal media anolyte (1500 mW/m2). These experiments demonstrate that power density per volume for a biofilm flow reactor MFC should be calculated using the anode chamber volume alone (250W/m3), rather than with the full anolyte volume. Two oxygen reduction cathodes (uncoated GF or a Pt/vulcanized carbon coating on GF) were also compared to a cathode using uncoated GF and a 50mM ferricyanide catholyte solution. The Pt/C-GF (2-4% Pt by mass) electrodes with liquid cultures of DSP10 produced one order of magnitude larger power density (150W/m3) than bare graphite felt (12W/m3) in this design. These advances are some of the required modifications to enable the mini-MFC to be used in real-time, long-term environmental power generating situations.
使用微型微生物燃料电池（mini-MFC，腔室体积：1.2 mL）监测在营养条件有限的情况下，嗜水气单胞菌DSP10纯培养物在石墨毡（GF）上形成生物膜的过程。当使用无细胞的基本培养基阳极电解液时，mini-MFC产生的可观功率密度（每装置横截面）（1500 mW/m2）支持了在GF上形成生物膜的环境扫描电子显微镜证据。这些实验表明，生物膜流动反应器MFC的单位体积功率密度应仅使用阳极腔室体积（250W/m3）来计算，而不是使用整个阳极电解液体积。还将两种氧还原阴极（未涂覆的GF或GF上的Pt/硫化碳涂层）与使用未涂覆的GF和50mM铁氰化钾阴极电解液的阴极进行了比较。在该设计中，带有DSP10液体培养物的Pt/C-GF（质量分数为2-4%的Pt）电极产生的功率密度（150W/m3）比裸石墨毡（12W/m3）大一个数量级。这些进展是使mini-MFC能够用于实时、长期环境发电情况所需的一些改进。
