Environmental Protection Department, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C/Profesor Albareda 1, 18008-Granada, Spain.
Microb Biotechnol. 2009 Mar;2(2):253-61. doi: 10.1111/j.1751-7915.2009.00084.x.
Bacterial membranes constitute the first physical barrier against different environmental stresses. Pseudomonas putida DOT-T1E accumulates cyclopropane fatty acids (CFAs) in the stationary phase of growth. In this strain the cfaB gene encodes the main cyclopropane synthase responsible of the synthesis of CFAs, and its expression is mediated by RNA polymerase with sigma factor σ(38). We generated a cfaB mutant of P. putida DOT-T1E and studied its response to solvents, acid pH and other stress conditions such as temperature changes, high osmolarity and the presence of antibiotics or heavy metals in the culture medium. A CfaB knockout mutant was more sensitive to solvent stress than the wild-type strain, but in contrast to Escherichia coli and Salmonella enterica, the P. putida cfaB mutant was as tolerant to acid shock as the wild-type strain. The cfaB mutant was also as tolerant as the parental strain to a number of drugs, antibiotics and other damaging agents.
细菌膜构成了抵御不同环境压力的第一道物理屏障。恶臭假单胞菌 DOT-T1E 在生长的静止期积累环丙烷脂肪酸 (CFA)。在该菌株中，cfaB 基因编码主要的环丙烷合酶，负责 CFA 的合成，其表达受 RNA 聚合酶和 sigma 因子 σ(38)介导。我们生成了恶臭假单胞菌 DOT-T1E 的 cfaB 突变体，并研究了它对溶剂、酸性 pH 值以及其他应激条件（如温度变化、高渗透压以及培养基中抗生素或重金属的存在）的反应。CfaB 敲除突变体比野生型菌株对溶剂应激更敏感，但与大肠杆菌和肠炎沙门氏菌不同，恶臭假单胞菌 cfaB 突变体对酸冲击的耐受性与野生型菌株相同。该 cfaB 突变体对许多药物、抗生素和其他损伤剂的耐受性与亲本菌株相同。
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