Wiener F, Silva S, Sugiyama H, Babonits M, Klein G
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Genes Chromosomes Cancer. 1990 May;2(1):36-43. doi: 10.1002/gcc.2870020107.
A reciprocal translocation between chromosomes 15 and 16 has been detected in seven murine plasmacytomas induced by a combination of pristane and Abelson virus. Six of the tumors were induced in a new, unconventional experimental system based on transfer of uninfected or Abelson virus infected bone marrow and spleen cells, respectively, into pristane treated mice. All six tumors were of donor type. The seventh tumor appeared in a conventional pristane + Abelson virus treated mouse. This tumor was unusual in carrying both the 15;16 variant translocation and the typical 12;15 translocation, in the same tumor cells. In the new 15;16 variant, the breakpoint of chromosome 15 was at the interphase of the D2/3 sub-bands, as in mouse plasmacytomas with the previously well-known typical 12;15 and variant 6;15 translocations. The breakpoint on chromosome 16 was mapped to band 16B1, corresponding to the presumed cytogenetic site of the Ig-lambda gene. In three of the seven tumors with the 15;16 translocation, the derivative chromosome 15 had undergone a duplication, a feature that has not been previously encountered in the MPC-associated 12;15 and 6;15 translocation carriers. The reciprocal derivative chromosome 16 was lost from one of the seven tumors. We postulate that the 15;16 translocation results in juxtaposition of the myc gene to lambda sequences, probably in a similar orientation as previously described for the variant 6;15 translocation.
在由 pristane 和 Abelson 病毒联合诱导的七例小鼠浆细胞瘤中检测到了 15 号和 16 号染色体之间的相互易位。其中六例肿瘤是在一个新的、非常规的实验系统中诱导产生的，该系统分别将未感染或感染 Abelson 病毒的骨髓和脾细胞转移到经 pristane 处理的小鼠体内。所有这六例肿瘤均为供体类型。第七例肿瘤出现在一只经传统的 pristane + Abelson 病毒处理的小鼠身上。这例肿瘤不同寻常之处在于，在同一肿瘤细胞中同时携带了 15;16 变异易位和典型的 12;15 易位。在新的 15;16 变异中，15 号染色体的断点位于 D2/3 亚带的间期，这与具有先前广为人知的典型 12;15 和变异 6;15 易位的小鼠浆细胞瘤情况相同。16 号染色体上的断点定位于 16B1 带，对应于 Ig-λ 基因的推测细胞遗传学位点。在七例具有 15;16 易位的肿瘤中有三例，衍生的 15 号染色体发生了重复，这是在与小鼠浆细胞瘤相关的 12;15 和 6;15 易位携带者中先前未曾遇到过的特征。七例肿瘤中的一例丢失了相互衍生的 16 号染色体。我们推测，15;16 易位导致 myc 基因与 λ 序列并列，其方向可能与先前描述的变异 6;15 易位相似。
