Cherif D, Berger R
INSERM U301, Institut de Génétique Moléculaire, Paris, France.
Genes Chromosomes Cancer. 1990 Jul;2(2):103-8. doi: 10.1002/gcc.2870020205.
The chromosomal localization of genes of three VH families (VH 1-3) was performed using in situ hybridization with biotinylated probes. Significantly strong signals were observed on chromosome 14, band 14q32, and on bands 16p11 and 15q11, although less frequently. Signal intensity and frequency were more important on chromosome 14 with all three probes, and on chromosome 16 with the VH2 and VH3 probes, while chromosome 15 was more marked than 16 with the VH1 probe. The localization of VH gene on chromosomes other than 14 suggests that several genes of the VH family had been simultaneously translocated in evolution and that the newly localized VH sequences may be pseudogenes.
利用生物素化探针进行原位杂交，对三个VH家族（VH 1 - 3）的基因进行染色体定位。在14号染色体14q32带以及16p11和15q11带上观察到明显较强的信号，不过出现频率较低。使用所有三种探针时，14号染色体上的信号强度和频率更为显著，使用VH2和VH3探针时16号染色体上的情况也是如此，而使用VH1探针时，15号染色体比16号染色体表现得更为明显。VH基因在14号染色体以外的其他染色体上的定位表明，VH家族的几个基因在进化过程中同时发生了易位，并且新定位的VH序列可能是假基因。
