生长锥颗粒膜的p38和p34多肽是G蛋白的α和β亚基。

The p38 and p34 polypeptides of growth cone particle membranes are the alpha- and beta-subunits of G proteins.

作者信息

Edmonds B T, Moomaw C R, Hsu J T, Slaughter C, Ellis L

机构信息

Howard Hughes Medical Institute, University of Texas Southwestern Medical School, Dallas 75235-9050.

出版信息

Brain Res Dev Brain Res. 1990 Oct 1;56(1):131-6. doi: 10.1016/0165-3806(90)90172-u.

DOI:10.1016/0165-3806(90)90172-u

PMID:2126227

Abstract

Growth cone particle (GCP) membranes prepared from fetal day 17 rat brain are comprised of 5 major polypeptides as analyzed by SDS-PAGE: tubulin (p52), actin (p42), pp46/GAP-43 and two unidentified species, p38 and p34. Antibodies specific for the alpha- and beta-subunits of G proteins recognize p38 and p34, respectively, on immunoblots following one- and two-dimensional electrophoretic separation. That G protein subunits comprise major species of GCP membrane-associated polypeptides suggests a role for G proteins in transmembrane signaling in nerve growth cones.

摘要

从胚胎第17天大鼠脑制备的生长锥颗粒（GCP）膜，经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析由5种主要多肽组成：微管蛋白（p52）、肌动蛋白（p42）、pp46/GAP-43以及两种未鉴定的蛋白，p38和p34。G蛋白α亚基和β亚基的特异性抗体，在一维及二维电泳分离后的免疫印迹中分别识别p38和p34。G蛋白亚基构成GCP膜相关多肽的主要种类，这表明G蛋白在神经生长锥的跨膜信号传导中发挥作用。