Department of Gene Function and Regulation, Institute of Life Sciences, Department of Biotechnology, Govt. of India, Nalco Square, Chandrasekherpur, Bhubaneswar 751 023, Orissa, India.
J Biotechnol. 2011 Mar 10;152(1-2):58-62. doi: 10.1016/j.jbiotec.2011.01.015. Epub 2011 Jan 22.
Addition of multiple repeats of the FS3 upstream activation sequence (FS3-UAS, -270 to -60) intra-molecularly to the TATA containing core-domain of the FS3 (-151 to +31) promoter resulted in 2-3-folds enhanced promoter activity. The chimeric promoter, FS3-UAS-3X with maximum activity, showed 3.31 times stronger activity in root vascular tissue compared to FS3 promoter and could be used efficiently in translational research.
FS3 启动子的 TATA 核心区域（-151 到 +31）内源性地加入多个 FS3 上游激活序列（FS3-UAS，-270 到 -60）重复，可使启动子活性增强 2-3 倍。具有最大活性的嵌合启动子 FS3-UAS-3X 在根维管束组织中的活性比 FS3 启动子强 3.31 倍，可在翻译研究中有效使用。
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