Maiti I B, Shepherd R J
Department of Agronomy and Plant Pathology, University of Kentucky, Lexington 40546-0236, USA.
Biochem Biophys Res Commun. 1998 Mar 17;244(2):440-4. doi: 10.1006/bbrc.1998.8287.
A promoter fragment from peanut chlorotic streak caulimovirus (PClSV) full-length transcript (FLt) was identified and later modified to have duplicated enhancer domain. The FLt promoter with its single or double enhancer domains, fused with the GUS reporter gene to form chimeric gene constructs, showed a high level of expression of these genes in cells and transgenic plants. The FLt promoter with its double enhancer domain gives an average threefold greater expression of genes compared to the FLt promoter with its single enhancer domain in transgenic plants. In young seedlings the expression was in the order root > leaf > stem. The histochemical GUS assay in young seedlings showed more activity in root tips and leaf midribs, veins, and other vascular tissues. The expression from the PClSV FLt promoter was compared with that from the figwort mosaic virus promoter in transgenic plants. These constitutive promoters were comparable in respect to GUS expression level.
一个来自花生褪绿条纹花椰菜花叶病毒（PClSV）全长转录本（FLt）的启动子片段被鉴定出来，随后进行了修饰，使其具有重复的增强子结构域。带有单个或双增强子结构域的FLt启动子，与GUS报告基因融合形成嵌合基因构建体，在细胞和转基因植物中显示出这些基因的高水平表达。与在转基因植物中带有单个增强子结构域的FLt启动子相比，带有双增强子结构域的FLt启动子使基因的平均表达量提高了三倍。在幼苗中，表达顺序为根＞叶＞茎。幼苗的组织化学GUS分析表明，根尖、叶中脉、叶脉和其他维管组织中的活性更高。在转基因植物中，将PClSV FLt启动子的表达与玄参花叶病毒启动子的表达进行了比较。这些组成型启动子在GUS表达水平方面具有可比性。
