Kim Ki Hean, Park B Hyle, Tu Yupeng, Hasan Tayyaba, Lee Byunghak, Li Jianan, de Boer Johannes F
Department of Mechanical Engineering and Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, Korea.
Opt Express. 2011 Jan 17;19(2):552-61. doi: 10.1364/OE.19.000552.
Polarization-sensitive optical coherence tomography (PS-OCT) is an augmented form of OCT, providing 3D images of both tissue structure and polarization properties. We developed a new method of polarization-sensitive optical frequency domain imaging (PS-OFDI), which is based on a wavelength-swept source. In this method the sample was illuminated with unpolarized light, which was composed of two orthogonal polarization states (i.e., separated by 180° in the Poincaré sphere) that are uncorrelated to each other. Reflection of these polarization states from within the sample was detected simultaneously and independently using a frequency multiplexing scheme. This simultaneous sample probing with two polarization states enabled determination of the depth-resolved Jones matrices of the sample. Polarization properties of the sample were obtained by analyzing the sample Jones matrices through eigenvector decomposition. The new PS-OFDI system ran at 31K wavelength-scans/s with 3072 pixels per wavelength-scan, and was tested by imaging a polarizer and several birefringent tissues such as chicken muscle and human skin. Lastly the new PS-OFDI was applied to imaging two cancer animal models: a mouse model by injecting cancer cells and a hamster cheek pouch model. These animal model studies demonstrated the significant differences in tissue polarization properties between cancer and normal tissues in vivo.
偏振敏感光学相干断层扫描（PS-OCT）是光学相干断层扫描（OCT）的一种增强形式，可提供组织结构和偏振特性的三维图像。我们开发了一种基于扫频光源的新型偏振敏感光学频域成像（PS-OFDI）方法。在该方法中，用非偏振光照射样品，该非偏振光由两个相互正交的偏振态（即在庞加莱球上相隔180°）组成，且这两个偏振态彼此不相关。使用频率复用方案同时且独立地检测样品内部这些偏振态的反射。用两个偏振态同时探测样品能够确定样品的深度分辨琼斯矩阵。通过特征向量分解分析样品琼斯矩阵来获得样品的偏振特性。新型PS-OFDI系统以每秒31000次波长扫描运行，每次波长扫描有3072个像素，并通过对偏振器以及几种双折射组织（如鸡肌肉和人体皮肤）成像进行了测试。最后，新型PS-OFDI被应用于对两种癌症动物模型成像：一种是通过注射癌细胞构建的小鼠模型，另一种是仓鼠颊囊模型。这些动物模型研究证明了体内癌症组织和正常组织在组织偏振特性方面存在显著差异。
