Yu Li-bo, Wang Jing, Ma Bao-zhang, Sun Wen-zhou
Affiliated Tumor Hospital of Harbin Medical University, Harbin 150081, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2010 Nov;41(6):986-8, 1038.
To study the effects of ursolic acid (UA) on the invasion and metastasis of ovarian carcinoma cell HO-8910PM and its underlying mechanism.
MTT assay was performed to examine the effects of UA on the proliferation of ovarian carcinoma cells HO-8910PM in vitro. The effects of UA on the invasion and migration of HO-8910PM were evaluated using Transwell chambers attached with polycarbonate filters and reconstituted basement membrane. Gelatin zymography was performed to detect the activity of gelatinase in the HO-8910PM cells. The expressions of MMP-2 and MMP-9 in the HO-8910PM cells were measured by Western blot.
UA inhibited the proliferation of HO-8910PM cells in a time- and dose-dependent manner. There was a statistically significant difference in the invasion and migration of HO-8910PM cells between the UA treated cells and the controls (P < 0.01, P < 0.05). UA inhibited the activity of gelatinase of the treated cells.
UA downregulated the expressions of MMP-2 and MMP-9. UA inhibits the invasion and metastasis of HO-8910PM cells, probably through inhibiting the activity of gelatinase and the expressions of MMP-2 and MMP-9.
研究熊果酸（UA）对卵巢癌细胞HO - 8910PM侵袭和转移的影响及其潜在机制。
采用MTT法检测UA对体外培养的卵巢癌细胞HO - 8910PM增殖的影响。使用附有聚碳酸酯滤膜和重组基底膜的Transwell小室评估UA对HO - 8910PM侵袭和迁移的影响。采用明胶酶谱法检测HO - 8910PM细胞中明胶酶的活性。通过蛋白质免疫印迹法检测HO - 8910PM细胞中MMP - 2和MMP - 9的表达。
UA以时间和剂量依赖性方式抑制HO - 8910PM细胞的增殖。UA处理组细胞与对照组相比，HO - 8910PM细胞的侵袭和迁移能力存在统计学显著差异（P < 0.01，P < 0.05）。UA抑制了处理组细胞明胶酶的活性。
UA下调MMP - 2和MMP - 9的表达。UA可能通过抑制明胶酶活性以及MMP - 2和MMP - 9的表达来抑制HO - 8910PM细胞的侵袭和转移。
