Li Ka, Yang Jie, Chen Zeng-rong
Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2010 Nov;41(6):1039-43.
To investigate the genetic polymorphisms of interleukin-1B (IL-1B) and interleukin-1 receptor antagonist gene (IL-1RN) in gastric cancer, and to explore the relationships of these genetic polymorphisms to the susceptibility of gastric cancer.
The polymorphisms of IL-1B and IL-1RN genes were analyzed by PCR-restriction fragment length polymorphism (PCR-RFLP) after extracting the genomic DNA from 140 gastric cancer patients and 165 age- and sex-matched healthy controls.
The polymorphisms of IL-1B promoter region -31, -511 and +3954 locus have no significant difference between gastric cancer patients and healthy subjects. Four kinds of polymorphisms of IL-1RN were found as 2R/2R, 2R/4R, 3R/4R and 4R/4R, and the frequency in gastric cancer patients were 0.7%, 15.7%, 2.9% and 80.7%, respectively, while the frequency in healthy controls were 0, 5.5%, 0 and 94.5%, respectively. Compared to 4R/4R genotype, a 3.37 fold increased risk of gastric cancer were found in 2R/4R genotype, but the difference was not significant (P = 0.557, chi2 = 2.076). IL-1RN 2R allele frequencies in gastric cancer and healthy controls were 8.6% and 2.7%, respectively, which showed the risk to be gastric cancer increased 3.4 times, but the difference was not significant (P = 0.781, chi2 = 0.494).
There is no evidence to support that the polymorphism of IL-1B and IL-1RN gene had relationship with gastric cancer. However, the risk of developing gastric cancer might be raised when the IL-1RN 2R allele exist.
研究白细胞介素-1β（IL-1β）和白细胞介素-1受体拮抗剂基因（IL-1RN）在胃癌中的基因多态性，并探讨这些基因多态性与胃癌易感性的关系。
从140例胃癌患者和165例年龄及性别匹配的健康对照者中提取基因组DNA，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术分析IL-1β和IL-1RN基因的多态性。
胃癌患者与健康受试者之间IL-1β启动子区-31、-511和+3954位点的多态性无显著差异。发现IL-1RN有2R/2R、2R/4R、3R/4R和4R/4R四种多态性，在胃癌患者中的频率分别为0.7%、15.7%、2.9%和80.7%，而在健康对照者中的频率分别为0、5.5%、0和94.5%。与4R/4R基因型相比，2R/4R基因型患胃癌的风险增加了3.37倍，但差异无统计学意义（P = 0.557，χ2 = 2.076）。胃癌患者和健康对照者中IL-1RN 2R等位基因频率分别为8.6%和2.7%，表明患胃癌的风险增加了3.4倍，但差异无统计学意义（P = 0.781，χ2 = 0.494）。
没有证据支持IL-1β和IL-1RN基因多态性与胃癌有关。然而，当存在IL-1RN 2R等位基因时，患胃癌的风险可能会增加。
