Singh H, Sachan R, Goel H, Mittal B
Department of Genetics, SGPGIMS, Lucknow, India.
BJOG. 2008 Apr;115(5):633-8. doi: 10.1111/j.1471-0528.2007.01655.x.
Inflammation plays a major role in pathogenesis of cervical cancer. We planned to study whether polymorphisms in inflammation-related genes, IL-1RN (VNTR) and IL-1beta (-511C/T), are associated with risk of cervical cancer.
Case-control study.
Uttar Pradesh state in India.
One hundred and fifty, histopathologically confirmed cases with cervical cancer and 162 age-, ethnicity-matched, cervical cytology negative, healthy controls were recruited to this study.
Genotyping of IL-1RN (VNTR) and IL-1beta (-511C/T) polymorphisms was performed using polymerase chain reaction (PCR)/PCR-restriction fragment length polymorphism. Power of study was 80% with type 1 error of 0.05. Haplotypes frequencies were obtained by computer package 'Arlequin'.
Haplotype IL-1RN2/IL-1betaT is associated with higher risk and of cervical cancer.
IL-1RN genotypes 1/2 and 2/2 were associated with significantly elevated risk of cervical cancer (OR = 3.3; P= 4.9 x 10(-6) and OR = 2.9, P= 0.02). Similarly, TT genotype of IL-1betapolymorphism was significantly higher in cases compared with controls (57.7 versus 38.3%; OR = 2.8; P = 0.012). 2/2 genotype of IL-1RN (OR = 4.8, P = 0.0006) and TT genotype of IL-1beta(OR = 5.2; P = 0.02) were associated with the higher stages (III) of cervical cancer. Haplotypes 1T (IL-1RN1/IL-1betaT) and 2T (IL-1RN2/IL-1betaT) were also significantly associated with higher susceptibility to cervical cancer and its progression. Logistic regression analysis suggests IL-1RN allele 2 and IL-1beta-511T were independently associated with increased risk for cervical cancer.
IL-1RN2 and IL-1beta -511T in various combinations of genotypes and haplotypes are associated with higher susceptibility for cervical cancer.
炎症在宫颈癌发病机制中起主要作用。我们计划研究炎症相关基因IL-1RN（可变数目串联重复序列）和IL-1β（-511C/T）的多态性是否与宫颈癌风险相关。
病例对照研究。
印度北方邦。
招募了150例经组织病理学确诊的宫颈癌病例和162例年龄、种族匹配、宫颈细胞学检查阴性的健康对照者参与本研究。
采用聚合酶链反应（PCR）/PCR限制性片段长度多态性方法对IL-1RN（可变数目串联重复序列）和IL-1β（-511C/T）多态性进行基因分型。研究效能为80%，I类错误为0.05。单倍型频率通过计算机软件“Arlequin”获得。
单倍型IL-1RN2/IL-1βT与宫颈癌风险较高相关。
IL-1RN基因分型1/2和2/2与宫颈癌风险显著升高相关（比值比[OR]=3.3；P=4.9×10⁻⁶和OR=2.9，P=0.02）。同样，与对照组相比，病例组中IL-1β多态性的TT基因型显著更高（57.7%对38.3%；OR=2.8；P=0.012）。IL-1RN的2/2基因型（OR=4.8，P=0.0006）和IL-1β的TT基因型（OR=5.2；P=0.02）与宫颈癌的较高分期（III期）相关。单倍型1T（IL-1RN1/IL-1βT）和2T（IL-1RN2/IL-1βT）也与宫颈癌易感性及其进展显著相关。逻辑回归分析表明，IL-1RN等位基因2和IL-1β-511T与宫颈癌风险增加独立相关。
IL-1RN2和IL-1β -511T在各种基因型和单倍型组合中与宫颈癌易感性较高相关。
