Ren Lin, Sun Cheng-jun, Zou Xiao-li, Zeng Hong-yan, Jiang Bo
Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2010 Nov;41(6):1055-8.
To develop a method for the rapid determination of ethanol in blood with capillary-GC.
0.50 mL of whole blood sample was taken and added with 1.00 mL of dimethyl sulfoxide (DMSO), and 2 g of anhydrous sodium sulfate. The supernatant of the sample solution was directly injected into GC for analysis.
Ethanol was separated from other substances in the sample. The liner range of ethanol detected by the capillary-GC was 0.0-300.0 mg/100 mL, and the detection limit was 0.2 mg/100 mL. The RSD for standard solution determination was 1.36%. Satisfactory results were obtained for the determination of ethanol in whole blood samples, with recoveries ranging from 90.9% to 107.3% and a RSD of 1.98%. The combined uncertainty was 2.2%.
This is a rapid, sensitive and simple method for determination of ethanol in large quantities of samples. The method has shortened the duration of analysis cycle in comparison with the traditional headspace-GC, with a reduction from 20-30 min to less than 10 min.
建立一种用毛细管气相色谱法快速测定血液中乙醇的方法。
取0.50 mL全血样品，加入1.00 mL二甲基亚砜（DMSO）和2 g无水硫酸钠。将样品溶液的上清液直接注入气相色谱仪进行分析。
乙醇与样品中的其他物质分离。毛细管气相色谱法检测乙醇的线性范围为0.0 - 300.0 mg/100 mL，检测限为0.2 mg/100 mL。标准溶液测定的相对标准偏差为1.36%。全血样品中乙醇的测定结果令人满意，回收率在90.9%至107.3%之间，相对标准偏差为1.98%。合成不确定度为2.2%。
这是一种快速、灵敏且简单的大量样品中乙醇测定方法。与传统顶空气相色谱法相比，该方法缩短了分析周期，从20 - 30分钟减少到不到10分钟。
