Department of Acupuncture and Moxibustion, College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
Int J Neurosci. 2011 Apr;121(4):209-17. doi: 10.3109/00207454.2010.548613. Epub 2011 Jan 26.
This study was designed to investigate the anti-inflammatory effects of bee venom (BV) in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of Parkinson's disease (PD).
MPTP was administered by intraperitoneal (IP) injection at 2-hr intervals over an 8-hr period. Mice were then subjected to BV subcutaneous injection and sacrificed on days 1 and 3 following the final MPTP injection. The loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc) was assessed by tyrosine hydroxylase (TH) immunohistochemistry. Microglial activation was measured by immunohistochemistry for macrophage antigen complex-1 (MAC-1) and inducible nitric oxide synthase (iNOS). The staining intensities of MAC-1 and iNOS were quantified with respect to optical density.
In animals treated with MPTP, the survival percentages of TH+ cells in the SNpc were 32% on day 1 and 46% on day 3 compared with normal mice. In BV-treated mice, the survival percentages of TH+ cells improved to 70% on day 1 and 78% on day 3 compared with normal mice. BV treatment also resulted in reduced expression of the inflammation markers MAC-1 and iNOS in the SNpc.
These data suggest that BV injection may have a neuroprotective effect that attenuates the activation of the microglial response, which has implications for the treatment of PD.
本研究旨在探讨蜂毒（BV）在 1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）诱导的帕金森病（PD）小鼠模型中的抗炎作用。
MPTP 通过腹腔（IP）注射，每 2 小时一次，共 8 小时。然后对小鼠进行 BV 皮下注射，并在最后一次 MPTP 注射后的第 1 天和第 3 天处死。通过酪氨酸羟化酶（TH）免疫组织化学评估黑质致密部（SNpc）中多巴胺能神经元的丢失。通过巨噬细胞抗原复合物-1（MAC-1）和诱导型一氧化氮合酶（iNOS）的免疫组织化学测量小胶质细胞的激活。MAC-1 和 iNOS 的染色强度相对于光密度进行量化。
在接受 MPTP 治疗的动物中，SNpc 中 TH+细胞的存活率在第 1 天为 32%，第 3 天为 46%，与正常小鼠相比。在 BV 治疗的小鼠中，TH+细胞的存活率在第 1 天提高到 70%，第 3 天提高到 78%，与正常小鼠相比。BV 治疗还导致 SNpc 中炎症标志物 MAC-1 和 iNOS 的表达减少。
这些数据表明，BV 注射可能具有神经保护作用，可减轻小胶质细胞反应的激活，这对 PD 的治疗具有重要意义。
