State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070 Wuhan, Hubei, China.
Biochem Soc Trans. 2011 Jan;39(1):99-103. doi: 10.1042/BST0390099.
Sulfolobus solfataricus and Sulfolobus islandicus contain several genes exhibiting D-arabinose-inducible expression and these systems are ideal for studying mechanisms of archaeal gene expression. At sequence level, only two highly conserved cis elements are present on the promoters: a regulatory element named ara box directing arabinose-inducible expression and the basal promoter element TATA, serving as the binding site for the TATA-binding protein. Strikingly, these promoters possess a modular structure that allows an essentially inactive basal promoter to be strongly activated. The invoked mechanisms include TFB (transcription factor B) recruitment by the ara-box-binding factor to activate gene expression and modulation of TFB recruitment efficiency to yield differential gene expression.
《嗜热硫还原球菌和硫矿硫化叶菌中含有几个表现出 D-阿拉伯糖诱导表达的基因，这些系统是研究古菌基因表达机制的理想模型。在序列水平上，启动子上只存在两个高度保守的顺式元件：一个名为 ara 盒的调控元件，指导阿拉伯糖诱导表达，以及作为 TATA 结合蛋白结合位点的基本启动子元件 TATA。引人注目的是，这些启动子具有模块化结构，允许基本无活性的基础启动子被强烈激活。所涉及的机制包括 ara 盒结合因子募集 TFB（转录因子 B）以激活基因表达，以及调节 TFB 募集效率以产生不同的基因表达。
