Biology Department and Center for Life in Extreme Environments, Portland State University, Portland, OR 97207, USA.
FEMS Microbiol Lett. 2011 Aug;321(2):92-9. doi: 10.1111/j.1574-6968.2011.02313.x. Epub 2011 Jun 6.
Ribosomal genes are strongly regulated dependent on growth phase in all organisms, but this regulation is poorly understood in Archaea. Moreover, very little is known about growth phase-dependent gene regulation in Archaea. SSV1-based lacS reporter gene constructs containing the Sulfolobus 16S/23S rRNA gene core promoter, the TF55α core promoter, or the native lacS promoter were tested in Sulfolobus solfataricus cells lacking the lacS gene. The 42-bp 16S/23S rRNA gene and 39-bp TF55α core promoters are sufficient for gene expression in S. solfataricus. However, only gene expression driven by the 16S/23S rRNA gene core promoter is dependent on the culture growth phase. This is the smallest known regulated promoter in Sulfolobus. To our knowledge, this is the first study to show growth phase-dependent rRNA gene regulation in Archaea.
核糖体基因在所有生物体中都强烈依赖于生长阶段进行调控，但这种调控在古菌中还了解甚少。此外，关于古菌中生长阶段依赖性基因调控的了解非常有限。在缺乏 lacS 基因的 Sulfolobus solfataricus 细胞中测试了基于 SSV1 的 lacS 报告基因构建体，其中包含 Sulfolobus 16S/23S rRNA 基因核心启动子、TF55α 核心启动子或天然 lacS 启动子。42bp 的 16S/23S rRNA 基因和 39bp 的 TF55α 核心启动子足以在 S. solfataricus 中进行基因表达。然而，只有由 16S/23S rRNA 基因核心启动子驱动的基因表达依赖于培养物的生长阶段。这是在 Sulfolobus 中已知的最小调控启动子。据我们所知，这是第一项表明古菌中 rRNA 基因调控依赖于生长阶段的研究。
