Giacomoni D, Ben-Efraim S, Najmabadi F, Dray S
Department of Microbiology and Immunology, University of Illinois, Chicago 60680.
Med Oncol Tumor Pharmacother. 1990;7(4):273-80. doi: 10.1007/BF02987106.
The concentrated supernatants of nine human melanoma cell line cultures were analyzed for the presence of factors that inhibit in vitro immunological reactions. All cell lines secreted a factor that inhibited LPS-induced proliferation of murine B cells; eight cell lines released a factor that inhibited PHA-induced proliferation of murine T cells; all of the three cell lines investigated secreted a factor that inhibited the allogeneic stimulation of BALB/c spleen cells by mitomycin-C-treated C57BL spleen cells. Further analysis of the M21 cell supernatant indicated that its continuous presence was required for the inhibition of the PHA but not of the LPS response suggesting a different mechanism of action. High levels of PHA, but not of LPS, could overcome the inhibitory effect of M21 supernatants. Fractionation of M21 supernatants by sucrose gradient centrifugation, DEAE chromatography and gel filtration suggested that the anti-PHA and the anti-LPS activities were due to different factors. These factors differed from a serine proteinase inhibitor that is also released by M21 cells. Since these inhibitory factors may have a role in protecting a melanoma tumor from attack by the immune system of the host, their consideration may be helpful in designing protocols for therapy which include methods to boost the antitumor responses of the host.
对9种人黑色素瘤细胞系培养物的浓缩上清液进行分析，以检测是否存在抑制体外免疫反应的因子。所有细胞系均分泌一种抑制小鼠B细胞LPS诱导增殖的因子；8种细胞系释放一种抑制小鼠T细胞PHA诱导增殖的因子；所研究的3种细胞系均分泌一种抑制丝裂霉素C处理的C57BL脾细胞对BALB/c脾细胞的同种异体刺激的因子。对M21细胞上清液的进一步分析表明，其持续存在是抑制PHA反应所必需的，但对LPS反应则不是，这表明其作用机制不同。高水平的PHA而非LPS能够克服M21上清液的抑制作用。通过蔗糖梯度离心、DEAE层析和凝胶过滤对M21上清液进行分级分离，结果表明抗PHA和抗LPS活性归因于不同的因子。这些因子不同于M21细胞也释放的一种丝氨酸蛋白酶抑制剂。由于这些抑制因子可能在保护黑色素瘤肿瘤免受宿主免疫系统攻击方面发挥作用，因此在设计包括增强宿主抗肿瘤反应方法的治疗方案时考虑这些因子可能会有所帮助。
