Gauthier-Rahman S, Suzuki K, Couderc J, el-Gharbi N, Decreusefond C, Stiffel C
Laboratoire de Biochimie (CNRS SDI-16201), Faculté de Pharmacie, Université de Paris-Sud, Châtenay-Malabry, France.
Exp Clin Immunogenet. 1991;8(3):159-76.
Expression of migration inhibition factor (MIF) following in vitro stimulation with phytohemagglutinin (PHA) or allogeneic cells was explored in two lines of mice genetically selected for the high (Hi/PHA) or low (Lo/PHA) response of their lymphoid cells to PHA. Hi/PHA mice also have greater cell-mediated immune responses in mixed lymphocyte culture and graft-versus-host reactions, the poorer cell-mediated immune response of Lo/PHA being accompanied by a higher frequency of malignant tumours. Expression of MIF in PHA-pulsed spleen cell supernatants measured by a sensitive photoelectric method was found to be modulated by the concomitant presence of migration stimulation factor (MStF) derived from T cells. Both lymphokines were better expressed in Lo/PHA, as compared to Hi/PHA, under appropriate experimental conditions. Use of a low proliferative dose of mitogen (5 micrograms/ml PHA, 2-hour pulse) followed by culture in serum-free medium led Lo/PHA to express the highest titres of MIF, whereas a proliferative dose of PHA (50 micrograms/ml, 2-hour pulse) caused abrogation or occultation of expression of MIF and elective expression of MStF in this line. Hi/PHA mice expressed MIF equally at both mitogen doses, with transient expression of MStF followed by MIF after 50 micrograms/ml PHA, the kinetics of expression of the two lymphokines being different. Expression of MStF by spleen cells was an early event after PHA stimulation. In contrast to mitogenic stimulation, allogeneic stimulation in one-way mixed lymphocyte culture led to similar expression of MIF by both lines of mice. The implications of these findings are discussed.
在两组经基因筛选的小鼠中，研究了用植物血凝素（PHA）或同种异体细胞进行体外刺激后，迁移抑制因子（MIF）的表达情况。这两组小鼠分别为其淋巴细胞对PHA反应高（Hi/PHA）或低（Lo/PHA）的品系。Hi/PHA小鼠在混合淋巴细胞培养和移植物抗宿主反应中也具有更强的细胞介导免疫反应，而Lo/PHA小鼠较差的细胞介导免疫反应伴随着更高频率的恶性肿瘤。通过灵敏的光电方法测定，发现PHA刺激的脾细胞上清液中MIF的表达受源自T细胞的迁移刺激因子（MStF）的共同存在调节。在适当的实验条件下，与Hi/PHA相比，Lo/PHA中两种淋巴因子的表达均更好。使用低增殖剂量的丝裂原（5微克/毫升PHA，2小时脉冲），然后在无血清培养基中培养，导致Lo/PHA表达最高滴度的MIF，而增殖剂量的PHA（50微克/毫升，2小时脉冲）导致该品系中MIF表达的消除或隐匿以及MStF的选择性表达。Hi/PHA小鼠在两种丝裂原剂量下均同等表达MIF，在50微克/毫升PHA后先短暂表达MStF，随后表达MIF，两种淋巴因子的表达动力学不同。脾细胞对MStF的表达是PHA刺激后的早期事件。与丝裂原刺激相反，单向混合淋巴细胞培养中的同种异体刺激导致两品系小鼠中MIF的表达相似。讨论了这些发现的意义。
