Franco-Czech Laboratory for Clinical Research on Obesity, Department of Sports Medicine, 3rd Faculty of Medicine, Charles University, Prague, Czech Republic.
Diabetologia. 2011 Apr;54(4):876-87. doi: 10.1007/s00125-010-2014-3. Epub 2011 Jan 26.
AIMS/HYPOTHESIS: Our goal was to identify a set of human adipose tissue macrophage (ATM)-specific markers and investigate whether their gene expression in subcutaneous adipose tissue (SAT) as well as in visceral adipose tissue (VAT) is related to obesity and to the occurrence of the metabolic syndrome.
ATM-specific markers were identified by DNA microarray analysis of adipose tissue cell types isolated from SAT of lean and obese individuals. We then analysed gene expression of these markers by reverse transcription quantitative PCR in paired samples of SAT and VAT from 53 women stratified into four groups (lean, overweight, obese and obese with the metabolic syndrome). Anthropometric measurements, euglycaemic-hyperinsulinaemic clamp, blood analysis and computed tomography scans were performed.
A panel of 24 genes was selected as ATM-specific markers based on overexpression in ATM compared with other adipose tissue cell types. In SAT and VAT, gene expression of ATM markers was lowest in lean and highest in the metabolic syndrome group. mRNA levels in the two fat depots were negatively correlated with glucose disposal rate and positively associated with indices of adiposity and the metabolic syndrome.
CONCLUSIONS/INTERPRETATION: In humans, expression of ATM-specific genes increases with the degree of adiposity and correlates with markers of insulin resistance and the metabolic syndrome to a similar degree in SAT and in VAT.
目的/假设：我们的目标是确定一组人类脂肪组织巨噬细胞（ATM）特异性标志物，并研究其在皮下脂肪组织（SAT）和内脏脂肪组织（VAT）中的基因表达与肥胖以及代谢综合征的发生是否有关。
通过对从瘦人和肥胖个体的 SAT 中分离出的脂肪组织细胞类型进行 DNA 微阵列分析，鉴定 ATM 特异性标志物。然后，我们在 53 名女性的 SAT 和 VAT 配对样本中通过逆转录定量 PCR 分析这些标志物的基因表达，这些女性分为四组（瘦、超重、肥胖和肥胖伴代谢综合征）。进行了人体测量、血糖正常-高胰岛素钳夹、血液分析和计算机断层扫描。
根据与其他脂肪组织细胞类型相比在 ATM 中的过度表达，选择了一组 24 个基因作为 ATM 特异性标志物。在 SAT 和 VAT 中，ATM 标志物的基因表达在瘦组中最低，在代谢综合征组中最高。两个脂肪储存部位的 mRNA 水平与葡萄糖处置率呈负相关，与肥胖和代谢综合征的指标呈正相关。
结论/解释：在人类中，ATM 特异性基因的表达随着肥胖程度的增加而增加，并且与 SAT 和 VAT 中胰岛素抵抗和代谢综合征的标志物具有相似的相关性。
