Cambridge Centre of Neuropsycharitic Research, Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
Proteomics. 2011 Feb;11(3):501-5. doi: 10.1002/pmic.201000525. Epub 2011 Jan 7.
In this study, we performed the first high-throughput and comprehensive proteomic profiling of the rat hippocampal proteome. Using a combination of 2-D LC-MS and data analysis with the Rosetta Elucidator(®) system, we identified 1340 unique proteins. Functional classification showed that most of these were associated with synaptic function and comprised a high proportion of phosphorylated proteins and analytically challenging classes of membrane proteins such as ion channel receptor subunits.
在这项研究中，我们对大鼠海马体蛋白质组进行了首次高通量和全面的蛋白质组学分析。通过 2-D LC-MS 联用技术和 Rosetta Elucidator（®）系统的数据分析，我们鉴定出了 1340 种独特的蛋白质。功能分类表明，其中大多数与突触功能相关，并且包含了大量磷酸化蛋白质和分析上具有挑战性的膜蛋白类别，如离子通道受体亚基。
