Nakipova O V, Averin A S, Zakharova N M, Uchitel' M L, Grishina E V, Bogdanova L A, Maevskiĭ E I
Biofizika. 2010 Nov-Dec;55(6):1124-31.
The effect of ambiocor (15 mg/100 ml), which contains natural substrates of energy metabolism, on the contractility of papillary muscles (PM) of the right ventricle of the rat heart was studied at stimulation frequencies from 0.1 to 3.0 Hz at a temperature of 30 +/- 1 degrees C (n = 7). The effect was recorded 20 min after the addition of the preparation. It was demonstrated that ambiocor causes a significant (about 70%), independent of stimulation frequency, suppression of the amplitude of isometric contractions (negative inotropic effect), which is coupled with an increase in the relative value of the rest potentiation effect (a qualitative index of calcium content in sarcoplasmic reticulum). The influence of the mixture leads to significant alterations in the time parameters of the "contraction-relaxation" cycle: an increase in the duration of latent period; and a decrease in the time to peak tension and half-relaxation time (TR50%). The effect of the mixture is partially reversible. During the washing of the preparation with the control solution, the qualitative indicators of the contractile activity of papillary muscles are substantially improved in comparison with the initial ones. The character of alterations allows one to assume that the effect of ambiocor in the papillary muscles of the rat heart is realized partly through the suppression of the activity of sarcolemmal calcium channels.
研究了含有能量代谢天然底物的安比奥可（15毫克/100毫升）在30±1摄氏度的温度下，以0.1至3.0赫兹的刺激频率对大鼠心脏右心室乳头肌（PM）收缩性的影响（n = 7）。在加入制剂20分钟后记录该效应。结果表明，安比奥可会引起显著的（约70%）、与刺激频率无关的等长收缩幅度抑制（负性变力作用），这与静息增强效应的相对值增加（肌浆网钙含量的定性指标）相关。该混合物的影响导致“收缩 - 舒张”周期的时间参数发生显著变化：潜伏期持续时间增加；达到峰值张力的时间和半舒张时间（TR50%）减少。该混合物的作用部分可逆。在用对照溶液冲洗制剂期间，与初始情况相比，乳头肌收缩活动的定性指标有显著改善。变化特征使人可以推测，安比奥可在大鼠心脏乳头肌中的作用部分是通过抑制肌膜钙通道的活性实现的。
