Department of Physiology and Pharmacology Vittorio Erspamer, Sapienza University of Rome, P. le Aldo Moro 5, 00185, Rome, Italy.
Br J Nutr. 2011 Jul;106(1):33-6. doi: 10.1017/S0007114510005763. Epub 2011 Jan 27.
Extra-virgin olive oil is an integral ingredient of the Mediterranean diet, and it has been suggested that its high consumption has beneficial effects on human health. Its protective effect, in particular against the development of CVD, has been related not only to the high content of oleic acid, but also to the antioxidant and anti-inflammatory properties of polyphenols. In order to verify the anti-inflammatory and anti-atherogenic properties of hydroxy-isochromans, a class of ortho-diphenols present in extra-virgin olive oil, we investigated the potential ability of 1-phenyl-6,7-dihydroxy-isochroman (L137) to modulate the production of key inflammatory mediators by human monocytes, by evaluating its in vitro effects on prostanoid (thromboxane A(2) and PGE(2)) and cytokine (TNF-α) production. Its effect on the protein expression of the inducible form of cyclo-oxygenase-2 (COX-2), a pro-inflammatory enzyme responsible for elevated prostanoid levels, was also explored. The results showed that L137 significantly inhibited both prostanoid and TNF-α production in lipopolysaccharide-primed human monocytes in a dose-dependent manner, by inhibiting the COX activity of COX-2. We also demonstrated that the effects of the isochroman are mediated, at least partly, through the suppression of NF-κB activation leading to the down-regulation of the synthesis of COX-2.
特级初榨橄榄油是地中海饮食的一个组成部分，有人认为其高摄入量对人类健康有益。它的保护作用，特别是对 CVD 的发展，不仅与油酸的高含量有关，而且与多酚的抗氧化和抗炎特性有关。为了验证羟基异黄酮类化合物（一种存在于特级初榨橄榄油中的邻二酚）的抗炎和抗动脉粥样硬化特性，我们研究了 1-苯基-6,7-二羟基异黄酮（L137）对人单核细胞关键炎症介质产生的潜在调节能力，通过评估其对前列腺素（血栓素 A2 和 PGE2）和细胞因子（TNF-α）产生的体外影响。还研究了其对诱导型环加氧酶-2（COX-2）的蛋白表达的影响，COX-2 是一种负责升高前列腺素水平的促炎酶。结果表明，L137 以剂量依赖的方式显著抑制脂多糖预刺激的人单核细胞中前列腺素和 TNF-α的产生，抑制 COX-2 的 COX 活性。我们还证明，异黄酮的作用至少部分是通过抑制 NF-κB 激活来介导的，从而下调 COX-2 的合成。
