Fungicide Research Group, Centre for Sustainable Pest and Disease Management, Department of Plant Pathology and Microbiology, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ , United Kingdom.
Int J Food Microbiol. 2011 Jan 31;145(1):370-4. doi: 10.1016/j.ijfoodmicro.2011.01.011. Epub 2011 Jan 12.
Contamination of cereals with mycotoxins such as beauvericin (BEA), enniatins (Ens) and moniliformin (MON) is mainly caused by Fusarium avenaceum and F. tricinctum. This is a world-wide problem which requires rapid and sensitive detection methods. To allow for high throughput screening of large numbers of samples, a diagnostic PCR method was developed for the simultaneous detection of F. avenaceum and F. tricinctum. The interspecific divergence found in the Fusarium-specific CYP51C gene was used to design species-specific PCR primers. The specificity of the assay was demonstrated for DNA samples extracted from a wide range of Fusarium species belonging to the Fusarium head blight (FHB) complex, as well as for naturally-infected grain samples. The PCR-amplified products were digested with the restriction enzyme XbaI to enable differentiation between F. avenaceum and F. tricinctum. This PCR- restriction fragment length polymorphism (RFLP) assay proved to be a simple and relatively inexpensive method highly suited for routine detection and identification of F. avenaceum and F. tricinctum in wheat samples.
真菌毒素如 beauvericin（BEA）、enniatins（Ens）和 moniliformin（MON）污染谷物主要是由镰刀菌属（Fusarium）的禾谷镰刀菌（F. avenaceum）和三线镰刀菌（F. tricinctum）引起的。这是一个全球性的问题，需要快速灵敏的检测方法。为了能够高通量筛选大量样品，开发了一种用于同时检测禾谷镰刀菌和三线镰刀菌的诊断 PCR 方法。在真菌特异性 CYP51C 基因中发现的种间差异被用来设计种特异性 PCR 引物。该检测方法的特异性已通过提取自属于镰刀菌头腐病（FHB）复合体的多种镰刀菌属的 DNA 样本以及天然感染的谷物样本得到了证明。PCR 扩增产物用限制酶 XbaI 进行消化，以区分禾谷镰刀菌和三线镰刀菌。该 PCR-限制性片段长度多态性（RFLP）检测法被证明是一种简单且相对廉价的方法，非常适合于小麦样品中禾谷镰刀菌和三线镰刀菌的常规检测和鉴定。
