Li Er-ran, Hong Xin, Liu Xia, Shang Jun-yi, Wang Bo, Wang Kun, Wang Qiu-yue, Kang Jian
Institute of Respiratory Diseases, First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Jan;31(1):28-32.
To investigate the impact of energy metabolism at the cellular level on the expression of the water channel protein aquaporin 1 (AQP1).
Balb/c mouse fibroblasts were incubated with iodoacetamide (IA) in vitro, and the changes in AQP1 expression were detected by immunoblotting and immunohistochemistry at 0, 4, and 6 h.
IA induced the expression of AQP1 at 4 and 6 h accompanied with cell death. Reverse transcription PCR showed an increased expression of AQP1 mRNA in the cells. AQP1 expression was also upregulated by the inhibitor of microtubule and cytochrome C oxidase.
A pretranslational regulation occurs in IA-induced AQP1 expression in mouse fibroblasts, and the up-regulated AQP1 accumulation is characterized by mitochondria-related energy dependence.
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