水通道蛋白1和3在小鼠前列腺中的表达及其意义

[Expressions of aquaporins 1 and 3 in the mouse prostate and their significance].

作者信息

Zhu Jian-Guo, Wu Mei, Liu Ya-Nan, Zhao Dan

机构信息

Department of Distinguished Professor Tang Ao-qing Laboratory, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, China.

出版信息

Zhonghua Nan Ke Xue. 2011 Jun;17(6):483-7.

PMID:21735643

Abstract

OBJECTIVE

To investigate the expressions of aquaporins (AQPs) in the mouse prostatic tissue and their significance, and to provide some evidence for a deeper insight into the physiological function and regulation of AQP expressions in normal and diseased prostatic tissues.

METHODS

The mRNA expressions of AQP0 - 4 in the mouse prostatic tissue were determined by RT-PCR, and the expressions and localizations of AQP1 and AQP3 proteins were characterized by Western blot and immunohistochemistry.

RESULTS

RT-PCR exhibited the mRNA expressions of AQP1 and AQP3, but not those of AQP0, AQP2 and AQP4 in the prostate tissue, while Western blot showed the expression of the AQP1 protein with the relative molecular mass (RMM) of 28 000 and those of the glycosylated and non-glycosylated AQP3 proteins with the RMM of 35 000 and 27 000, respectively. Immunohistochemistry indicated the strong expression of AQP1 in the cyst and plasma membrane of the secretary cells and that of AQP3 in the stroma cells of the prostate.

CONCLUSION

The AQP1 and AQP3 genes were expressed in the secretary epithelia of the mouse prostate tissue, which suggests that AQP1 and AQP3 may play an important role in the secretion of prostatic fluid.

摘要

目的

研究水通道蛋白（AQPs）在小鼠前列腺组织中的表达及其意义，为深入了解正常及病变前列腺组织中AQP表达的生理功能和调控提供依据。

方法

采用逆转录聚合酶链反应（RT-PCR）检测小鼠前列腺组织中AQP0 - 4的mRNA表达，并用蛋白质印迹法和免疫组织化学法对AQP1和AQP3蛋白的表达及定位进行鉴定。

结果

RT-PCR显示前列腺组织中有AQP1和AQP3的mRNA表达，但无AQP0、AQP2和AQP4的表达；蛋白质印迹法显示有相对分子质量（RMM）为28 000的AQP1蛋白表达，以及RMM分别为35 000和27 000的糖基化和非糖基化AQP3蛋白表达。免疫组织化学表明AQP1在分泌细胞的胞质和质膜中高表达，AQP3在前列腺基质细胞中高表达。