[骨髓间充质干细胞对衰老大鼠免疫功能的影响]

[Effect of bone-marrow mesenchymal stem cells on the immune function of aging rats].

作者信息

Wang Zhi-hong, Guo Kun-yuan, Yang Yu-lian, Liu Kun, Li Jie, Zhou Xue-yun, He Qing-mei

机构信息

Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2011 Jan;31(1):146-50.

PMID:21269978

Abstract

OBJECTIVE

To investigate the effect of transplantation of bone-marrow mesenchymal stem cells (MSCs) on the immune functions of aging rats.

METHODS

Healthy SD rats were randomized into normal control, aging model group and MSCs group. The aging model was established by daily subcutaneous injection of D-galactose for 4 consecutive months. MSCs were isolated from the bone marrow of adult SD rats and injected (3×10(6) MSCs) in rats in the MSCs group via the tail vein once a week for 4 weeks. The spleen index, activity of T lymphocytes and the levels of IL-2 and IL-10 in spleen were measured, and the pathological changes of the spleen were observed after the treatments.

RESULTS

MSCs transplantation enhanced the cellular immune function of aging rats manifested by obviously increased spleen index, activity of T lymphocyte and the level of IL-2, and lowered level of IL-10 in the spleen. The rats in the aging model group showed serious spleen injury, which was obviously lessened by MSCs injection.

CONCLUSION

MSCs transplantation can improve the cellular immune function of aging rats and ameliorate spleen injury induced by D-galactose.

摘要

目的

探讨骨髓间充质干细胞（MSCs）移植对衰老大鼠免疫功能的影响。

方法

将健康SD大鼠随机分为正常对照组、衰老模型组和MSCs组。通过连续4个月每日皮下注射D-半乳糖建立衰老模型。从成年SD大鼠骨髓中分离MSCs，并于MSCs组大鼠中每周经尾静脉注射一次（3×10(6)个MSCs），共注射4周。检测脾脏指数、T淋巴细胞活性以及脾脏中IL-2和IL-10水平，并观察处理后脾脏的病理变化。