Wang Zhihong, Lin Yun, Chen Weimin, Shang Jin, Wei Tiannan
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017 Feb;33(2):151-4.
To investigate the impact of bone marrow mesenchymal stem cell( BMSC) transplantation on the antioxidant capacity and immune activity of aging rats induced by D-galactose.
Ten healthy male SD rats served as a control group( aged 2 months). To establish aging models,healthy SD rats were daily injected subcutaneously with D-galactose( 400 mg / kg). Then the aging model rats were randomized into aging model group and BMSC group( ten rats in each group). The BMSC group was injected with 3 × 106 BMSCs via tail vein. And rats in the control and model groups were injected with the same amount of normal saline. Blood samples were taken from the rats of the three groups to detect the content of malonaldehyde( MDA) and the activities of superoxide dismutase( SOD). The thymic mass was weighed and the indexes of thymus were calculated; thymus lymphocyte transforming index was measured with MTT assay; the levels of IL-2and IL-10 in the thymus were detected by ELISA; and the ultrastructural changes of the thymus in each group were observed under a transmission electron microscope.
BMSC transplantation can increase the activity of SOD,decrease the level of MDA. Compared with the model group,the indexes of thymus as well as thymus lymphocyte transforming index significantly increased in the BMSC group. And in the BMSC group,the level of IL-2 was higher,and the level of IL-10 was distinctly lower. The thymus cells were arranged loosely,some nuclei presented with characteristic changes of pyknosis or apoptosis,and adipose tissues increased in the aging model group. BMSC could protect the ultrastructures of thymus cells,reticulo-epithelial cells,and the cell organelles were abundant and complete.
BMSC transplantation can improve antioxidant capacity and immune activity of aging rats,thus postponing immunosenescence.
探讨骨髓间充质干细胞（BMSC）移植对D-半乳糖诱导的衰老大鼠抗氧化能力及免疫活性的影响。
选取10只健康雄性SD大鼠作为对照组（2月龄）。将健康SD大鼠每日皮下注射D-半乳糖（400mg/kg）以建立衰老模型。然后将衰老模型大鼠随机分为衰老模型组和BMSC组（每组10只）。BMSC组经尾静脉注射3×10⁶个BMSC。对照组和模型组大鼠注射等量生理盐水。采集三组大鼠血液样本，检测丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性。称量胸腺质量并计算胸腺指数；采用MTT法检测胸腺淋巴细胞转化指数；用ELISA法检测胸腺中白细胞介素-2（IL-2）和白细胞介素-10（IL-10）水平；在透射电子显微镜下观察各组胸腺的超微结构变化。
BMSC移植可提高SOD活性，降低MDA水平。与模型组相比，BMSC组胸腺指数及胸腺淋巴细胞转化指数显著升高。且BMSC组IL-2水平较高，IL-10水平明显较低。衰老模型组胸腺细胞排列疏松，部分细胞核呈现固缩或凋亡特征性改变，脂肪组织增多。BMSC可保护胸腺细胞、网状上皮细胞的超微结构，细胞器丰富且完整。
BMSC移植可提高衰老大鼠的抗氧化能力及免疫活性，从而延缓免疫衰老。
