Iwama Y
Department of Anatomy, Aichi Medical University, Japan.
Okajimas Folia Anat Jpn. 1990 Oct;67(4):281-7. doi: 10.2535/ofaj1936.67.4_281.
Mouse pituitary gonadotropes were identified using immunohistochemical techniques with OsO4 single fixation. Most gonadotropes are immunostained with both LH beta and FSH antisera (FSH/LH gonadotropes), whereas a few gonadotropes are positive only for LH beta (LH gonadotropes). The former gonadotropes are large rounded or oval cells and contain two types of secretory granules: electron-dense smaller ones (about 100-200 nm in diameter) and electron-transparent larger ones (maximum diameter, about 400 nm) enclosed by a halo. The endoplasmic reticulum appears as a series of dilated sacs, and the Golgi apparatus is distinct. The mitochondria are found to have an electron-dense matrix throughout the cytoplasm, but are often concentrated in and around the region of the Golgi apparatus. These fine structural features are somewhat similar to those of the classical Barnes "FSH cell" The LH gonadotropes are polygonal cells of medium size. Small granules, as described above, are seen, but large granules are not. The Golgi apparatus is distinct, but small. The LH gonadotropes resemble neither the Barnes "LH cell" nor the Kurosumi-Oota "LH cell".
采用四氧化锇单次固定的免疫组织化学技术鉴定小鼠垂体促性腺激素细胞。大多数促性腺激素细胞同时被促黄体生成素β（LHβ）和促卵泡生成素（FSH）抗血清免疫染色（FSH/LH促性腺激素细胞），而少数促性腺激素细胞仅对LHβ呈阳性（LH促性腺激素细胞）。前者促性腺激素细胞为大的圆形或椭圆形细胞，含有两种类型的分泌颗粒：电子致密的较小颗粒（直径约100 - 200nm）和被晕圈包围的电子透明的较大颗粒（最大直径约400nm）。内质网表现为一系列扩张的囊泡，高尔基体明显。线粒体在整个细胞质中具有电子致密的基质，但通常集中在高尔基体区域及其周围。这些精细结构特征与经典的巴恩斯“FSH细胞”有些相似。LH促性腺激素细胞是中等大小的多边形细胞。可见上述的小颗粒，但未见大颗粒。高尔基体明显，但较小。LH促性腺激素细胞既不像巴恩斯“LH细胞”，也不像黑须 - 大田“LH细胞”。
