Nakamura F, Taya K, Sasamoto S, Yoshimura F
Acta Anat (Basel). 1985;124(1-2):104-10. doi: 10.1159/000146103.
Morphological and functional changes of pituitary LH/FSH cells in the female rat were investigated using the parameters on the radioimmunoassay, immunohistochemistry and ultrastructure. Changes in immunostainability, populations of intensely immunostained LH and FSH cells and total volume of secretory granules were correlated with the changes in pituitary LH and FSH contents during the estrous cycle. The immunohistochemical feature of gonadotropin release is the transformation of intensely immunostained gonadotrophs into the weakly stained ones. Secretory granules of small diameter (less than 150 nm) were numerous just before LH and FSH surges then sharply declined along with LH and FSH surges. The number of secretory granules of large diameter (larger than 150 nm) also decreased when LH and FSH surges took place. Then the number increased progressively until 17.00 h on the day of diestrus, corresponding to the increase in pituitary LH and FSH contents. It is suggested that small secretory granules are a release pool while large ones are a reserve pool.
利用放射免疫测定、免疫组织化学和超微结构等参数，研究了雌性大鼠垂体促黄体生成素/促卵泡生成素（LH/FSH）细胞的形态和功能变化。动情周期中，促黄体生成素（LH）和促卵泡生成素（FSH）细胞免疫染色性的变化、强免疫染色的LH和FSH细胞数量以及分泌颗粒的总体积与垂体LH和FSH含量的变化相关。促性腺激素释放的免疫组织化学特征是强免疫染色的促性腺细胞转变为弱染色的细胞。直径小（小于150nm）的分泌颗粒在LH和FSH峰前数量众多，随后随着LH和FSH峰的出现而急剧下降。LH和FSH峰出现时，直径大（大于150nm）的分泌颗粒数量也减少。然后数量逐渐增加，直到动情后期的17:00，这与垂体LH和FSH含量的增加相对应。提示小分泌颗粒是释放池，而大分泌颗粒是储备池。
