Bryon P A, Lagarde M, Dechavanne M
Nouv Rev Fr Hematol (1978). 1978 Jun 10;20(2):173-8.
Stereologic study of the in vitro release reaction from unstirred washed platelets provided quantitative information on the contractile wave induced by thrombin. Contraction was estimated from the reduction in the volume of both the granular compartment and the vacuoles of the surface connected canalicular system. Dibutyryl cyclic-AMP (10(-4) M) and dipyridamole (10(-3) M) inhibited degranulation, but they had no effect on the contraction produced by thrombin at high concentrations (0.1 UI/ml). Aspirin (10(-4) M) inhibited contraction induced by thrombin at concentration of 0.1 and 0.02 UI/ml (p less than 0.01 at each concentration). In two patients with hereditary cyclo-oxygenase deficiency, contraction produced by thrombin at low concentration (0.02 UI/ml) failed to induce contraction.
对未搅拌的洗涤血小板体外释放反应的体视学研究提供了有关凝血酶诱导的收缩波的定量信息。通过颗粒区室和表面连接小管系统的液泡体积减少来估计收缩。二丁酰环磷腺苷（10(-4) M）和双嘧达莫（10(-3) M）抑制脱颗粒，但它们对高浓度（0.1 UI/ml）凝血酶产生的收缩没有影响。阿司匹林（10(-4) M）在浓度为0.1和0.02 UI/ml时抑制凝血酶诱导的收缩（每种浓度下p均小于0.01）。在两名遗传性环氧化酶缺乏症患者中，低浓度（0.02 UI/ml）凝血酶产生的收缩未能诱导收缩。
