Harry S. Truman Memorial Veterans' Hospital, Columbia, Missouri, USA.
Nanomedicine. 2011 Aug;7(4):505-13. doi: 10.1016/j.nano.2011.01.006. Epub 2011 Jan 25.
This study examined the gene transfer efficiency and toxicity of 2-kDa polyethylenimine conjugated to gold nanoparticles (PEI2-GNPs) in the human cornea in vitro and rabbit cornea in vivo. PEI2-GNPs with nitrogen-to-phosphorus ratios of up to 180 exhibited significant transgene delivery in the human cornea without altering the viability or phenotype of these cells. Similarly, PEI2-GNPs applied to corneal tissues collected after 12 hours, 72 hours, or 7 days exhibited appreciable gold uptake throughout the rabbit stroma with gradual clearance of GNPs over time. Transmission electron microscopy detected GNPs in the keratocytes and the extracellular matrix of the rabbit corneas. Additionally, slit-lamp biomicroscopy in live animals even 7 days after topical PEI2-GNP application to the cornea detected no inflammation, redness, or edema in rabbit eyes in vivo, with only moderate cell death and immune reactions. These results suggest that PEI2-GNPs are safe for the cornea and can potentially be useful for corneal gene therapy in vivo.
This study examined the gene transfer efficiency and toxicity of 2-kDa polyethylenimine conjugated to gold nanoparticles in the human cornea in vitro and rabbit cornea in vivo. The results suggest that PEI2-GNPs are safe for the cornea and can potentially be useful for corneal gene therapy in vivo.
本研究在体外人角膜和体内兔角膜中检查了与金纳米粒子偶联的 2kDa 聚乙稀亚胺（PEI2-GNPs）的基因转移效率和毒性。氮磷比高达 180 的 PEI2-GNPs 在人角膜中表现出显著的转基因传递，而不改变这些细胞的活力或表型。同样，在收集到的角膜组织上应用于 12 小时、72 小时或 7 天后的 PEI2-GNPs ，在整个兔基质中表现出可观的金摄取，随着时间的推移逐渐清除 GNPs。透射电子显微镜在兔角膜的角膜细胞和成纤维细胞中检测到 GNPs。此外，在活体动物的裂隙灯生物显微镜检查中，即使在将 PEI2-GNPs 局部应用于角膜 7 天后，在体内兔眼中也未检测到炎症、发红或水肿，只有中度的细胞死亡和免疫反应。这些结果表明，PEI2-GNPs 对角膜是安全的，并且可能对体内角膜基因治疗有用。
本研究在体外人角膜和体内兔角膜中检查了与金纳米粒子偶联的 2kDa 聚乙稀亚胺（PEI2-GNPs）的基因转移效率和毒性。结果表明，PEI2-GNPs 对角膜是安全的，并且可能对体内角膜基因治疗有用。
