Jin Sha, Leach John C, Ye Kaiming
College of Engineering, University of Arkansas, Fayetteville, AR, USA.
Methods Mol Biol. 2009;544:547-57. doi: 10.1007/978-1-59745-483-4_34.
Nonviral gene delivery has been gaining considerable attention recently. Although the efficacy of DNA transfection, which is a major concern, is low in nonviral vector-mediated gene transfer compared with viral ones, nonviral vectors are relatively easy to prepare, less immunogenic and oncogenic, and have no potential of virus recombination and no limitation on the size of a transferred gene. The ability to incorporate genetic materials such as plasmid DNA, RNA, and siRNA into functionalized nanoparticles with little toxicity demonstrates a new era in pharmacotherapy for delivering genes selectively to tissues and cells. In this chapter, we highlight the basic concepts and applications of nonviral gene delivery using super paramagnetic iron oxide nanoparticles and functionalized silica nanoparticles. The experimental protocols related to these topics are described in the chapter.
近年来，非病毒基因递送受到了广泛关注。尽管作为主要关注点的DNA转染效率在非病毒载体介导的基因转移中与病毒载体相比很低，但非病毒载体相对易于制备，免疫原性和致癌性较低，没有病毒重组的潜在风险，并且对转移基因的大小没有限制。将质粒DNA、RNA和siRNA等遗传物质整合到低毒性的功能化纳米颗粒中的能力，为将基因选择性地递送至组织和细胞的药物治疗开创了一个新时代。在本章中，我们重点介绍了使用超顺磁性氧化铁纳米颗粒和功能化二氧化硅纳米颗粒进行非病毒基因递送的基本概念和应用。与这些主题相关的实验方案在本章中进行了描述。
