从一只患有B细胞淋巴瘤的猫建立一种新型的猫白血病病毒（FeLV）阴性B细胞系。
Establishment of a novel feline leukemia virus (FeLV)-negative B-cell cell line from a cat with B-cell lymphoma.
作者信息
Mochizuki Hiroyuki, Takahashi Masashi, Nishigaki Kazuo, Ide Tetsuya, Goto-Koshino Yuko, Watanabe Shinya, Sato Hirofumi, Sato Masahiko, Kotera Yukiko, Fujino Yasuhito, Ohno Koichi, Uchida Kazuyuki, Tsujimoto Hajime
机构信息
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
出版信息
Vet Immunol Immunopathol. 2011 Apr 15;140(3-4):307-11. doi: 10.1016/j.vetimm.2010.12.010. Epub 2011 Jan 8.
We established a novel feline B-cell line, MS4, from the neoplastic pleural effusion of a cat with cutaneous B-cell lymphoma. Immunophenotype staining of the MS4 cells was positive for CD20, CD79α, and IgA and negative for CD3, CD4, CD5, CD8α, CD18, CD21, CD22, IgM, IgG, Ig light chain, and MHC class II. PCR analysis for immunoglobulin heavy chain gene rearrangements revealed a monoclonal rearrangement, whereas no clonal rearrangement of the T-cell receptor γ gene was detected. Southern blotting with an exogenous feline leukemia virus (FeLV) U3 probe revealed no integration of exogenous FeLV provirus. The MS4 cell line is the first FeLV-negative feline B-cell lymphoma cell line, and may be used to investigate the pathogenesis of spontaneously occurring feline lymphoma and the development of new therapies.
我们从一只患有皮肤B细胞淋巴瘤的猫的肿瘤性胸腔积液中建立了一种新型猫B细胞系MS4。MS4细胞的免疫表型染色显示CD20、CD79α和IgA呈阳性，而CD3、CD4、CD5、CD8α、CD18、CD21、CD22、IgM、IgG、Ig轻链和MHC II类呈阴性。免疫球蛋白重链基因重排的PCR分析显示为单克隆重排，而未检测到T细胞受体γ基因的克隆重排。用外源性猫白血病病毒（FeLV）U3探针进行的Southern印迹分析显示没有外源性FeLV前病毒的整合。MS4细胞系是首个FeLV阴性的猫B细胞淋巴瘤细胞系，可用于研究自发性猫淋巴瘤的发病机制以及新疗法的开发。
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