Jackson M L, Wood S L, Misra V, Haines D M
Department of Veterinary Pathology, Western College of Veterinary Medicine, Saskatoon, Saskatchewan.
Can J Vet Res. 1996 Jul;60(3):199-204.
The lymphocyte phenotype of 70 formalin-fixed, paraffin-embedded feline lymphosarcomas (LSAs) was determined immunohistochemically using a T cell polyclonal antibody, and a B cell monoclonal antibody. Forty-seven of 70 (67%) tumors were T cell, 19/70 (27%) were B cell, and 4/70 (6%) did not stain with either marker. Thirty-eight of 70 (54%) tumors were positive for feline leukemia virus (FeLV) antigen by immunohistochemistry (IHC), and 52/70 (74%) tumors were positive for FeLV DNA using the polymerase chain reaction (PCR). B cell tumors were as frequently FeLV-positive as T cell tumors using either IHC or PCR. Intestinal tumors were more likely to be B cell than T. The incidence of B and T cell tumors was not different among young (< or = 3 y), middle-aged (> 3 y to < or = 8 y), and old (> 8 y) cats. Both B and T cell tumors from old cats were FeLV-positive more often by PCR than by IHC. Feline leukemia virus DNA but not antigen, was detected in B cell tumors and intestinal tumors from cats > 8 y as often as it was detected in B cell tumors and intestinal tumors from cats < or = 8 y. Previously, most B cell and intestinal tumors from old cats were considered to be negative for FeLV. Here, the results suggest involvement of latent or replication-defective forms of the virus in such tumors from old cats. This study supports a role for FeLV in feline B cell as well as T cell tumorigenesis.
采用T细胞多克隆抗体和B细胞单克隆抗体，通过免疫组织化学方法确定了70例福尔马林固定、石蜡包埋的猫淋巴瘤（LSA）的淋巴细胞表型。70例肿瘤中，47例（67%）为T细胞型，19/70例（27%）为B细胞型，4/70例（6%）两种标记均未染色。70例肿瘤中，38例（54%）通过免疫组织化学（IHC）检测猫白血病病毒（FeLV）抗原呈阳性，52/70例（74%）肿瘤使用聚合酶链反应（PCR）检测FeLV DNA呈阳性。使用IHC或PCR检测时，B细胞肿瘤与T细胞肿瘤的FeLV阳性率相同。肠道肿瘤更可能是B细胞型而非T细胞型。在年轻（≤3岁）、中年（>3岁至≤8岁）和老年（>8岁）猫中，B细胞和T细胞肿瘤的发生率没有差异。老年猫的B细胞和T细胞肿瘤通过PCR检测FeLV阳性的频率高于IHC检测。在>8岁猫的B细胞肿瘤和肠道肿瘤中检测到FeLV DNA的频率与≤8岁猫的B细胞肿瘤和肠道肿瘤中检测到的频率一样高，但抗原检测结果并非如此。以前，大多数老年猫的B细胞和肠道肿瘤被认为FeLV呈阴性。在此，结果表明潜伏或复制缺陷形式的病毒参与了老年猫此类肿瘤的发生。这项研究支持FeLV在猫B细胞以及T细胞肿瘤发生中的作用。
