Fujino Yasuhito, Ma Zhiyong, Satoh Hitoshi, Mizuno Takuya, Hisasue Masaharu, Baba Kenji, Masuda Kenichi, Ohno Koichi, Onishi Takafumi, Tsujimoto Hajime
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Science, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Vet Immunol Immunopathol. 2004 Dec 28;102(4):429-39. doi: 10.1016/j.vetimm.2004.08.009.
A feline lymphoblastoid cell line (KO-1) was established from a 5-year-old neutered female cat with naturally occurring thymic lymphoma. KO-1 cells had a rearrangement of T-cell receptor beta-chain gene and a germ-line configuration of immunoglobulin heavy chain gene, however, they were devoid of T-cell-specific surface phenotype. Cytogenetically, KO-1 cells showed a hyperploidy (2n = 41) due to the trisomy of B2, F2 and X chromosomes. Although KO-1 cells were shown to be clonally expanded cells integrated with feline leukemia virus (FeLV) proviruses and expressed its structural proteins in their cytoplasm, they did not produce virus particles as shown by transmission electron microscopy and the absence of the viral protein and reverse transcriptase activity in the culture supernatant. The present study showed that the KO-1 cell line established here was a feline T-cell lymphoma cell line having a unique characteristic as an FeLV nonproducer.
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