Department of Plant Systems Biology, VIB, Technologiepark 927, B-9052 Gent, Belgium.
J Exp Bot. 2011 May;62(8):2571-83. doi: 10.1093/jxb/erq426. Epub 2011 Jan 27.
CLE peptides are potentially involved in nodule organ development and in the autoregulation of nodulation (AON), a systemic process that restricts nodule number. A genome-wide survey of CLE peptide genes in the soybean glycine max genome resulted in the identification of 39 GmCLE genes, the majority of which have not yet been annotated. qRT-PCR analysis indicated two different nodulation-related CLE expression patterns, one linked with nodule primordium development and a new one linked with nodule maturation. Moreover, two GmCLE gene pairs, encoding group-III CLE peptides that were previously shown to be involved in AON, had a transient expression pattern during nodule development, were induced by the essential nodulation hormone cytokinin, and one pair was also slightly induced by the addition of nitrate. Hence, our data support the hypothesis that group-III CLE peptides produced in the nodules are involved in primordium homeostasis and intertwined in activating AON, but not in sustaining it.
CLE 肽可能参与结节器官发育和自身调节的结瘤（AON），这是一个限制结瘤数量的系统过程。对大豆 Glycine max 基因组中的 CLE 肽基因进行全基因组调查，结果鉴定出 39 个 GmCLE 基因，其中大多数尚未被注释。qRT-PCR 分析表明，两种不同的与结瘤相关的 CLE 表达模式，一种与结节原基发育有关，另一种与结节成熟有关。此外，两对编码 III 组 CLE 肽的 GmCLE 基因对，先前被证明参与 AON，在结瘤发育过程中有一个短暂的表达模式，被必需的结瘤激素细胞分裂素诱导，一对也被硝酸盐略微诱导。因此，我们的数据支持这样的假设，即在结节中产生的 III 组 CLE 肽参与原基的动态平衡，并交织在激活 AON 中，但不在维持它。
