Suchi Kentaro, Fujiwara Hitoshi, Okamura Shinichi, Okamura Hiroko, Umehara Seiji, Todo Momoko, Furutani Akinobu, Yoneda Masayuki, Shiozaki Atsushi, Kubota Takeshi, Ichikawa Daisuke, Okamoto Kazuma, Otsuji Eigo
Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kawaramachi-hirokoji, Kamigyo-ku, Kyoto, Japan.
Anticancer Res. 2011 Jan;31(1):67-75.
Interleukin-6 (IL-6) expression at local tumor sites or in systemic circulation is associated with disease progression and poor prognosis of esophageal cancer. The aim of this study was to investigate the possible influence of IL-6 on biological activities of esophageal cancer cells in terms of chemosensitivity. Human esophageal cancer cell lines TE13 and KYSE170 were transfected with a plasmid vector expressing IL-6 and stable transfectants overexpressing IL-6 were thus established. The sensitivity of IL-6 transfectants to cisplatin was evaluated using a WST-8 assay and cell-cycle analysis. In addition, the inhibitory effects of IL-6-specific siRNAs were investigated. IL-6 transfectants showed significantly reduced sensitivity to cisplatin compared to control transfectants. In addition, the reduced cisplatin sensitivity of IL-6 transfectants was restored by pretreatment with IL-6-specific siRNA. These results suggest that intracellular IL-6 expression in tumor cells may acts as a resistance factor against cisplatin-based treatments for esophageal cancer.
白细胞介素-6（IL-6）在局部肿瘤部位或全身循环中的表达与食管癌的疾病进展和不良预后相关。本研究的目的是从化疗敏感性方面探讨IL-6对食管癌细胞生物学活性的可能影响。用人食管癌细胞系TE13和KYSE170转染表达IL-6的质粒载体，从而建立了过表达IL-6的稳定转染子。使用WST-8测定法和细胞周期分析评估IL-6转染子对顺铂的敏感性。此外，还研究了IL-6特异性siRNA的抑制作用。与对照转染子相比，IL-6转染子对顺铂的敏感性显著降低。此外，用IL-6特异性siRNA预处理可恢复IL-6转染子降低的顺铂敏感性。这些结果表明，肿瘤细胞内IL-6的表达可能作为食管癌基于顺铂治疗的耐药因子。
