Tong Qiang, Wang Xiao-Long, Li Sheng-Bao, Yang Gong-Li, Jin Shu, Gao Zi-Ye, Liu Xiao-Bo
Department of Gastroenterology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, People's Republic of China,
Department of Gastroenterology, Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, People's Republic of China.
Onco Targets Ther. 2018 Sep 12;11:5777-5787. doi: 10.2147/OTT.S171242. eCollection 2018.
The diagnosis rate of early stage esophageal squamous cell carcinoma (ESCC) is low due to the lack of specific tumor markers. Seeking for these markers is beneficial to improve the early diagnosis rate and the prognosis of patients. This study profiles the differentially expressed proteins of early stage ESCC patients via the AAH-BLG-507 protein chip, which further consolidates the clinical evidence of ESCC diagnosis.
In this study, 20 serum samples were collected from Taihe Hospital between August 2016 and June 2017. Ten of them carried ESCC, while the rest were healthy controls. To profile the proteins' expression level, the AAH-BLG-507 protein chip was used, and both highly expressed and lowly expressed proteins were fished out. Meanwhile, their biological roles were examined by using Gene Ontology (GO) database and String database, and they were further verified by ELISA.
Results showed that the expression levels of AXL, ARTN, Ang2, BDNF, BMP7, cripto-1, CCL28, E-selectin, IL-6, IL-8 and SHH in the serum of early ESCC were significantly upregulated (<0.05), particularly IL-6 and IL-8. The expression levels of TSP1 and MMP-8 were markedly downregulated (<0.05). Analysis showed that these proteins were mainly involved in angiogenesis, signal transduction, cell proliferation and migration, indicating the close relationship with the development of ESCC.
It suggested that IL-6 and IL-8 proteins could be considered as the markers for ESCC diagnosis.
由于缺乏特异性肿瘤标志物，早期食管鳞状细胞癌（ESCC）的诊断率较低。寻找这些标志物有助于提高患者的早期诊断率和预后。本研究通过AAH-BLG-507蛋白芯片分析早期ESCC患者差异表达的蛋白质，进一步巩固了ESCC诊断的临床证据。
本研究于2016年8月至2017年6月从太和医院收集了20份血清样本。其中10份患有ESCC，其余为健康对照。为分析蛋白质的表达水平，使用了AAH-BLG-507蛋白芯片，筛选出高表达和低表达的蛋白质。同时，通过基因本体论（GO）数据库和String数据库研究它们的生物学作用，并通过酶联免疫吸附测定（ELISA）进一步验证。
结果显示，早期ESCC患者血清中AXL、ARTN、Ang2、BDNF、BMP7、cripto-1、CCL28、E-选择素、IL-6、IL-8和SHH的表达水平显著上调（<0.05），尤其是IL-6和IL-8。TSP1和MMP-8的表达水平明显下调（<0.05）。分析表明，这些蛋白质主要参与血管生成、信号转导、细胞增殖和迁移，表明与ESCC的发生发展密切相关。
提示IL-6和IL-8蛋白可作为ESCC诊断的标志物。
