由黑色素瘤相关抗原（MAGE）启动子去甲基化介导的MAGE表达可诱导非小细胞肺癌进展。

MAGE expressions mediated by demethylation of MAGE promoters induce progression of non-small cell lung cancer.

作者信息

Yanagawa Naoki, Tamura Gen, Oizumi Hiroyuki, Endoh Makoto, Motoyama Teiichi

机构信息

Department of Pathology, Yamagata University Faculty of Medicine, Yamagata, Japan.

出版信息

Anticancer Res. 2011 Jan;31(1):171-5.

PMID:21273595

Abstract

BACKGROUND

The MAGE gene encodes cancer/testis antigens that are recognized on melanoma cells by autologous cytolytic T lymphocytes. These genes are expressed in various tumor cells, but not in healthy tissues except for the testis and placenta. MAGE expression is known to be activated by promoter demethylation.

MATERIALS AND METHODS

The expression of MAGE-A1 and -A3 and promoter methylation of MAGE-A1 and -A3 was investigated in 67 non-small cell lung cancer (NSCLC) specimens and their correlation with clinicopathological parameters was elucidated.

RESULTS

Expression of MAGE-A1 and -A3 was detected in 29.9% and 38.8% of the cases. Demethylation of MAGE-A1 and -A3 was detected in 41.8% and 46.3% of the cases. In 18 (of 20) cases, MAGE-A1 expression showed demethylation of MAGE-A1 and in 24 (of 26) cases MAGE-A3 expression showed demethylation of MAGE-A3. The patients with MAGE expression had a worse prognosis than those with no MAGE expression.

CONCLUSION

MAGE expression mediated by demethylation of MAGE promoters is associated with aggressive progression of NSCLC.

摘要

背景

MAGE基因编码癌症/睾丸抗原，自体溶细胞性T淋巴细胞可在黑色素瘤细胞上识别这些抗原。这些基因在各种肿瘤细胞中表达，但除睾丸和胎盘外，在健康组织中不表达。已知MAGE表达可通过启动子去甲基化激活。

材料与方法

研究了67例非小细胞肺癌（NSCLC）标本中MAGE-A1和-A3的表达以及MAGE-A1和-A3的启动子甲基化情况，并阐明了它们与临床病理参数的相关性。

结果

29.9%的病例检测到MAGE-A1表达，38.8%的病例检测到MAGE-A3表达。41.8%的病例检测到MAGE-A1去甲基化，46.3%的病例检测到MAGE-A3去甲基化。在20例中的18例中，MAGE-A1表达显示MAGE-A1去甲基化，在26例中的24例中，MAGE-A3表达显示MAGE-A3去甲基化。MAGE表达的患者预后比无MAGE表达的患者差。