Zhang Siya, Zhai Xiaolu, Wang Gui, Feng Jian, Zhu Huijun, Xu Liqin, Mao Guoxin, Huang Jianfei
Department of Chemotherapy, Nantong University Affiliated Hospital Nantong 226001, Jiangsu, China.
Department of Respiratory, Nantong University Affiliated Hospital Nantong 226001, Jiangsu, China.
Int J Clin Exp Pathol. 2015 Jan 1;8(1):541-50. eCollection 2015.
Melanoma associated antigen-A (MAGE-A) is an oncogene and correlated with tumor initiation and development. However the roles of MAGE-A9 in non-small cell lung cancer (NSCLC) are still unknown. We investigated MAGE-A9 mRNA expression in 18 tumor tissues of NSCLC by qRT-PCR and MAGE-A9 protein expression in 213 NSCLC samples of tissue arrays by immunohistochemical staining. We assessed the relationship between MAGE-A9 expression and clinical parameters. The results showed that the high expression of MAGE-A9 protein in NSCLC tumor cells were commonly present in squamous cell carcinomas (P = 0.030). It was also related to larger tumor diameter, lymph node metastasis and later stage grouping with TNM classification (all P < 0.05). Whereas the expression of MAGE-A9 in stromal cells was higher in squamous cell carcinomas as well. Cox regression univariate and multivariable analysis revealed that MAGE-A9 expression in tumor cells of NSCLC (P < 0.001) is an independent prognostic factor in five-year overall survival rate. We concluded that the molecular assessment of MAGEA9 could be considered to improve prognostic evaluation and to identify eligible patients for potential target therapy.
黑色素瘤相关抗原-A（MAGE-A）是一种癌基因，与肿瘤的发生和发展相关。然而，MAGE-A9在非小细胞肺癌（NSCLC）中的作用仍不清楚。我们通过qRT-PCR检测了18例NSCLC肿瘤组织中MAGE-A9 mRNA的表达，并通过免疫组织化学染色检测了213例NSCLC组织芯片样本中MAGE-A9蛋白的表达。我们评估了MAGE-A9表达与临床参数之间的关系。结果显示，NSCLC肿瘤细胞中MAGE-A9蛋白的高表达常见于鳞状细胞癌（P = 0.030）。它还与更大的肿瘤直径、淋巴结转移以及TNM分类的晚期分组相关（所有P < 0.05）。而MAGE-A9在基质细胞中的表达在鳞状细胞癌中也较高。Cox回归单因素和多因素分析显示，NSCLC肿瘤细胞中MAGE-A9的表达（P < 0.001）是五年总生存率的独立预后因素。我们得出结论，MAGEA9的分子评估可被认为有助于改善预后评估，并识别适合潜在靶向治疗的患者。
