Takeda Kayoko, Kinoshita Ichiro, Shimizu Yasushi, Matsuno Yoshihiro, Shichinohe Toshiaki, Dosaka-Akita Hirotoshi
Department of Medical Oncology, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.
Anticancer Res. 2011 Jan;31(1):263-70.
The Wnt target LGR5 has been recently identified as a murine intestinal stem cell marker. Its role during each stage of human colorectal tumorigenesis remains to be determined.
LGR5 expression was investigated by immunohistochemical analysis in 17 low-grade and 13 high-grade intraepithelial neoplasias and 30 adenocarcinomas.
The number of LGR5-positive cells increased tumor, with clustering of the cells to form patches. An apparent difference in their distribution among the tumorigenesis stages was observed. LGR5 expression in luminal surface showed a negative association with the progressive grade of tumors, while that in lower crypt exhibited a positive association with grade. In adenocarcinomas, LGR5 expression in luminal surface was negatively associated with pStage, while it was almost invariably high in lower crypt during advanced pStage disease.
These results suggest that the shifts in the distribution of LGR5-positive cells towards the lower crypt play a role in the development and progression of colorectal cancer.
Wnt靶标LGR5最近被确定为小鼠肠道干细胞标志物。其在人类结直肠癌发生各阶段中的作用仍有待确定。
通过免疫组化分析研究了17例低级别和13例高级别上皮内瘤变以及30例腺癌中LGR5的表达情况。
LGR5阳性细胞数量随肿瘤增大而增加，细胞聚集形成斑块。观察到其在肿瘤发生各阶段的分布存在明显差异。管腔表面的LGR5表达与肿瘤进展分级呈负相关，而在隐窝下部则与分级呈正相关。在腺癌中，管腔表面的LGR5表达与病理分期呈负相关，而在晚期病理分期疾病中，隐窝下部的LGR5表达几乎始终较高。
这些结果表明，LGR5阳性细胞分布向隐窝下部的转变在结直肠癌的发生和发展中起作用。
