CD133 和 LGR5 在溃疡性结肠炎相关结直肠癌及异型增生中的免疫组织化学表达。
Immunohistochemical Expression of CD133 and LGR5 in Ulcerative Colitis-associated Colorectal Cancer and Dysplasia.
机构信息
Division of Gastroenterological Surgery, Saitama Cancer Center, Saitama, Japan
Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, The University of Tokyo, Tokyo, Japan.
出版信息
In Vivo. 2019 Jul-Aug;33(4):1279-1284. doi: 10.21873/invivo.11600.
BACKGROUND/AIM: Cluster of differentiation 133 (CD133) and leu cine-rich orphan G-protein-coupled receptor 5 (LGR5) are the most putative stem cell markers for colorectal cancer (CRC), and are associated with poor prognosis of patients with CRC. However, the role of CD133 and LGR5 in the inflammation-dysplasia-carcinoma sequence has not been fully elucidated. We examined the expression of CD133 and LGR5 in ulcerative colitis-associated CRC (UC-CRC; n=20) and UC-associated colorectal dysplasia (n=16) by immunohistochemistry.
RESULTS
The rate of CD133-positive cases in UC-CRC was significantly higher than that in dysplasia (p=0.026), but that of LGR5 expression was not. Moreover, LGR5 expression was significantly positively associated with p53 expression (p=0.03), whereas CD133 expression positively correlated with p53 expression, but not significantly (p=0.10).
CONCLUSION
CD133 may play an important role in tumor development in the context of the inflammation-dysplasia-carcinoma sequence. LGR5-positive cancer stem cells may play a critical role in the development of UC-CRC, particularly upon loss of p53 function.
背景/目的：CD133 和富含亮氨酸的孤儿 G 蛋白偶联受体 5（LGR5）是结直肠癌（CRC）最具推测性的干细胞标志物，与 CRC 患者的预后不良相关。然而，CD133 和 LGR5 在炎症-发育不良-癌序列中的作用尚未完全阐明。我们通过免疫组织化学方法检测了溃疡性结肠炎相关结直肠癌（UC-CRC；n=20）和 UC 相关结直肠发育不良（n=16）中 CD133 和 LGR5 的表达。
结果
UC-CRC 中 CD133 阳性病例的发生率明显高于发育不良（p=0.026），但 LGR5 表达的发生率没有。此外，LGR5 表达与 p53 表达呈显著正相关（p=0.03），而 CD133 表达与 p53 表达呈正相关，但不显著（p=0.10）。
结论
CD133 可能在炎症-发育不良-癌序列中在肿瘤发展中起重要作用。LGR5 阳性癌症干细胞可能在 UC-CRC 的发生发展中发挥关键作用，特别是在 p53 功能丧失时。
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