Collins M S, Ladehoff D K, Mehton N S, Noonan J S
Cutter Biological, Miles Inc., Berkeley, CA 94701.
FEMS Microbiol Immunol. 1990 Dec;2(5-6):263-8. doi: 10.1111/j.1574-6968.1990.tb03528.x.
International Antigen Typing Schema (IATS) serotypes 1, 2, 5, 6, 8 and 11 comprise approximately 80% of Pseudomonas aeruginosa strains isolated from blood, wounds and respiratory specimens. Five human immunoglobulin M (IgM) monoclonal antibodies (MAbs) reactive with lipopolysaccharide O antigens of these IATS serotypes were studied in an opsonophagocytic assay. The assay employed human polymorphonuclear leukocytes, 2% guinea pig serum as the complement source and MAb. Each MAb promoted killing of inoculum of the homologous LPS serotype. The opsonic activity of each MAb was complement-dependent. In a murine model of Pseudomonas burn wound sepsis the LD50 of five strains of P. aeruginosa was increased greater than or equal to 22-fold by MAb-treatment (1.0 mg/kg). The mean effective dose of the five MAbs in mice challenged with approximately 10 LD50 of the homologous LPS serotype ranged from less than 0.01 mg/kg to 1.00 mg/kg.
国际抗原分型方案（IATS）血清型1、2、5、6、8和11约占从血液、伤口和呼吸道标本中分离出的铜绿假单胞菌菌株的80%。在吞噬调理试验中研究了五种与这些IATS血清型的脂多糖O抗原反应的人免疫球蛋白M（IgM）单克隆抗体（MAb）。该试验使用人多形核白细胞、2%豚鼠血清作为补体来源和单克隆抗体。每种单克隆抗体都能促进同源LPS血清型接种物的杀灭。每种单克隆抗体的调理活性都依赖于补体。在铜绿假单胞菌烧伤创面脓毒症的小鼠模型中，单克隆抗体治疗（1.0 mg/kg）使五株铜绿假单胞菌的半数致死量（LD50）增加了22倍或更多。在用大约10倍LD50的同源LPS血清型攻击的小鼠中，五种单克隆抗体的平均有效剂量范围为小于0.01 mg/kg至1.00 mg/kg。
