Hatano K, Goldberg J B, Pier G B
Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.
Infect Immun. 1995 Jan;63(1):21-6. doi: 10.1128/iai.63.1.21-26.1995.
Virulent strains of Pseudomonas aeruginosa are either of a nonmucoid, lipopolysaccharide (LPS)-smooth or mucoid, LPS-rough phenotype, and immunity to these different variants is efficiently mediated by antibodies specific to O antigens or mucoid exopolysaccharide (also called alginate), respectively. In addition to O side chains and core polysaccharide components, the LPS of P. aeruginosa also contains neutral-polysaccharide components that express antigenic determinants common to many clinical isolates. We evaluated antibodies specific to neutral polysaccharides for the ability to mediate opsonic killing and protective immunity. Antibodies to these antigens mediated opsonic killing of poorly virulent nonmucoid LPS-rough isolates but not of isogenic strains with either a LPS-smooth or a mucoid phenotype. Antibodies to neutral-polysaccharide antigens also failed to protect neutropenic mice from challenge with modest doses of LPS-smooth P. aeruginosa strains (< 10(3) CFU per mouse), whereas O-antigen-specific antibodies were highly protective. Antibodies to neutral polysaccharides deposited significantly (P = 0.002) more C3 onto LPS-rough strains than did antibodies to O side chains, but this situation was reversed when isogenic LPS-smooth strains were tested. Given that protective immunity against P. aeruginosa must be directed against either nonmucoid LPS-smooth strains or mucoid LPS-rough strains, it appears that antibodies specific to neutral-polysaccharide antigens do not protect against P. aeruginosa infection. Lack of protection is likely due to the ability of both O side chains and mucoid exopolysaccharide (alginate) to interfere with the opsonic killing activity of neutral-polysaccharide-specific antibodies.
铜绿假单胞菌的强毒株具有非黏液型、脂多糖（LPS）光滑型或黏液型、LPS粗糙型表型，针对这些不同变体的免疫分别由O抗原或黏液型胞外多糖（也称为藻酸盐）特异性抗体有效介导。除了O侧链和核心多糖成分外，铜绿假单胞菌的LPS还含有中性多糖成分，这些成分表达许多临床分离株共有的抗原决定簇。我们评估了针对中性多糖的抗体介导调理吞噬杀伤和保护性免疫的能力。针对这些抗原的抗体介导了对低毒力非黏液型LPS粗糙分离株的调理吞噬杀伤，但对具有LPS光滑型或黏液型表型的同基因菌株则没有作用。针对中性多糖抗原的抗体也未能保护中性粒细胞减少的小鼠免受中等剂量LPS光滑型铜绿假单胞菌菌株（每只小鼠<10³CFU）的攻击，而O抗原特异性抗体则具有高度保护作用。针对中性多糖的抗体比针对O侧链的抗体在LPS粗糙菌株上沉积的C3显著更多（P = 0.002），但在测试同基因LPS光滑菌株时情况则相反。鉴于针对铜绿假单胞菌的保护性免疫必须针对非黏液型LPS光滑菌株或黏液型LPS粗糙菌株，看来针对中性多糖抗原的抗体不能预防铜绿假单胞菌感染。缺乏保护作用可能是由于O侧链和黏液型胞外多糖（藻酸盐）都能够干扰中性多糖特异性抗体的调理吞噬杀伤活性。
