Niederhoffer E C, Fee J A
Stable Isotope Resource, Los Alamos National Laboratory, NM 87545.
Biol Met. 1990;3(3-4):237-41. doi: 10.1007/BF01140586.
In Escherichia coli, the superoxide dismutase genes (sodA and sodB) sense the availability of Fe through the action of the fur locus [E. C. Niederhoffer, C. M. Naranjo, K. L. Bradley, J. A. Fee (1990) Control of Escherichia coli superoxide dismutases (sodA and sodB) genes by the ferric uptake regulation (fur) locus, J. Bacteriol. 172, 1930-1938]. Previous work from other laboratories has shown that a variety of metal chelators and of redox-active aromatic compounds can dramatically induce expression of sodA. Here we show that non-redox-active, non-metal-chelating aromatic compounds also enhance expression of a chromosomal sodA gene fusion and that these effects are strongly modulated by the Fur phenotype (Fur +/-) and by the availability of iron in the culture medium. The compounds studied were ethidium bromide, hemin, 2,2'-bipyridine, 1,10-phenantroline, 4,7-phenantroline, rhodamine B1, rhodamine 6G, and, for comparison to previous studies, Paraquat.
在大肠杆菌中，超氧化物歧化酶基因（sodA和sodB）通过铁摄取调节基因座（fur）的作用来感知铁的可用性[E.C.尼德霍夫、C.M.纳兰霍、K.L.布拉德利、J.A.费伊（1990年）铁摄取调节（fur）基因座对大肠杆菌超氧化物歧化酶（sodA和sodB）基因的控制，《细菌学杂志》172卷，第1930 - 1938页]。其他实验室之前的研究表明，多种金属螯合剂和氧化还原活性芳香化合物能显著诱导sodA的表达。在此我们表明，非氧化还原活性、非金属螯合的芳香化合物也能增强染色体sodA基因融合体的表达，并且这些效应受到Fur表型（Fur +/-）和培养基中铁可用性的强烈调节。所研究的化合物有溴化乙锭、血红素、2,2'-联吡啶、1,10 - 菲咯啉、4,7 - 菲咯啉、罗丹明B1、罗丹明6G，以及为与之前研究作比较的百草枯。
