Kogoma T, Farr S B, Joyce K M, Natvig D O
Department of Cell Biology, School of Medicine, University of New Mexico, Albuquerque 87131.
Proc Natl Acad Sci U S A. 1988 Jul;85(13):4799-803. doi: 10.1073/pnas.85.13.4799.
Mu dX phage was used to isolate three gene fusions to the lacZ gene (soi::lacZ; soi for superoxide radical inducible) that were induced by treatment with superoxide radical anion generators such as paraquat and plumbagin. The induction of beta-galactosidase in these fusion strains with the superoxide radical generating agents required aerobic metabolism. Hyperoxygenation (i.e., bubbling of cultures with oxygen gas) also induced the fusions. On the other hand, hydrogen peroxide did not induce the fusions at concentrations that are known to invoke an adaptive response. Introduction of oxyR, htpR, or recA mutations did not affect the induction. Two of the fusion strains exhibited increased sensitivity to paraquat but not to hydrogen peroxide. The third fusion strain showed no increased sensitivity to either agent. All three fusions were located in the 45- to 61-min region of the Escherichia coli chromosome.
用Mu dX噬菌体分离出三个与lacZ基因的基因融合体（soi::lacZ；soi代表超氧自由基诱导型），这些融合体可由百草枯和白花丹素等超氧自由基阴离子生成剂处理诱导产生。在这些融合菌株中，超氧自由基生成剂诱导β-半乳糖苷酶的产生需要有氧代谢。过度氧化（即用氧气鼓泡培养物）也能诱导这些融合体。另一方面，已知能引发适应性反应的过氧化氢浓度却不能诱导这些融合体。引入oxyR、htpR或recA突变不影响诱导作用。其中两个融合菌株对百草枯的敏感性增加，但对过氧化氢不敏感。第三个融合菌株对这两种试剂的敏感性均未增加。所有三个融合体都位于大肠杆菌染色体的45至61分钟区域。
