H5ts125腺病毒DNA在体外的温度敏感性复制
Temperature-sensitive replication of H5ts125 adenovirus DNA in vitro.
作者信息
Horwitz M S
出版信息
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4291-5. doi: 10.1073/pnas.75.9.4291.
Abstract
A soluble adenovirus DNA replication system has been prepared in extracts of HeLa cells infected with the temperature-sensitive mutant H5ts125. These nuclear extracts synthesize full-sized viral DNA at 30 degrees but are temperature sensitive in this function at 38 degrees. A 72,000-dalton DNA-binding protein purified from cells infected with wild-type virus complements the temperature-sensitive defect and restores viral DNA replication to 74% of normal values.
摘要
已在感染温度敏感突变体H5ts125的HeLa细胞提取物中制备了一种可溶性腺病毒DNA复制系统。这些核提取物在30℃时合成全长病毒DNA，但在该功能上对38℃温度敏感。从感染野生型病毒的细胞中纯化出的一种72000道尔顿的DNA结合蛋白可弥补温度敏感缺陷，并将病毒DNA复制恢复至正常值的74%。
Zotero 插件