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H5ts125腺病毒DNA在体外的温度敏感性复制

Temperature-sensitive replication of H5ts125 adenovirus DNA in vitro.

作者信息

Horwitz M S

出版信息

Proc Natl Acad Sci U S A. 1978 Sep;75(9):4291-5. doi: 10.1073/pnas.75.9.4291.

DOI:10.1073/pnas.75.9.4291
PMID:212742
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336099/
Abstract

A soluble adenovirus DNA replication system has been prepared in extracts of HeLa cells infected with the temperature-sensitive mutant H5ts125. These nuclear extracts synthesize full-sized viral DNA at 30 degrees but are temperature sensitive in this function at 38 degrees. A 72,000-dalton DNA-binding protein purified from cells infected with wild-type virus complements the temperature-sensitive defect and restores viral DNA replication to 74% of normal values.

摘要

已在感染温度敏感突变体H5ts125的HeLa细胞提取物中制备了一种可溶性腺病毒DNA复制系统。这些核提取物在30℃时合成全长病毒DNA，但在该功能上对38℃温度敏感。从感染野生型病毒的细胞中纯化出的一种72000道尔顿的DNA结合蛋白可弥补温度敏感缺陷，并将病毒DNA复制恢复至正常值的74%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d8/336099/952fea4731f5/pnas00668-0230-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d8/336099/952fea4731f5/pnas00668-0230-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4d8/336099/952fea4731f5/pnas00668-0230-a.jpg

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In vitro complementation as an assay for purification of adenovirus DNA replication proteins.体外互补作为腺病毒DNA复制蛋白纯化的一种检测方法。
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Intermediates in the synthesis of type 2 adenovirus deoxyribonucleic acid.2型腺病毒脱氧核糖核酸合成中的中间体
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