Sussenbach J S, Kuijk M G, van Maarschalkerweerd M W
J Gen Virol. 1979 Jul;44(1):25-35. doi: 10.1099/0022-1317-44-1-25.
KB cells were infected with H5ts36 or H5ts125, two adenovirus type 5 (Ad5) mutants with a temperature-sensitive synthesis of virus DNA. Infection was started at the nonpermissive temperature and at 16 h p.i. the temperature was shifted down to the permissive temperature. Shortly after the shift-down H5ts125-infected cells showed an accumulation of purely single-stranded DNA of virus origin, which was not observed in H5ts36-infected cells. This single-stranded DNA has been characterized by hybridization and sedimentation analysis. It was found that the single-stranded DNA was derived from both complementary strands and consisted of short fragments. The observation that the single-stranded DNA accumulates in H5ts125-infected cells under conditions in which the amount of DNA binding protein is reduced, suggests that the DNA-binding protein is not only involved in initiation, but also in elongation of nascent strands.
KB细胞用H5ts36或H5ts125进行感染，这两种腺病毒5型（Ad5）突变体在病毒DNA合成方面具有温度敏感性。感染在非允许温度下开始，感染后16小时将温度降至允许温度。温度降低后不久，H5ts125感染的细胞显示出病毒来源的纯单链DNA积累，而在H5ts36感染的细胞中未观察到这种情况。这种单链DNA已通过杂交和沉降分析进行了表征。发现单链DNA来自两条互补链，并且由短片段组成。在DNA结合蛋白量减少的条件下，H5ts125感染的细胞中积累单链DNA的观察结果表明，DNA结合蛋白不仅参与起始，还参与新生链的延伸。
