Gibson Emily A, Masihzadeh Omid, Lei Tim C, Ammar David A, Kahook Malik Y
Department of Bioengineering, University of Colorado Denver, Denver, 12700 E. 19th Ave, Mail Stop 8607, Aurora, CO 80045, USA.
J Ophthalmol. 2011;2011:870879. doi: 10.1155/2011/870879. Epub 2011 Jan 3.
We review multiphoton microscopy (MPM) including two-photon autofluorescence (2PAF), second harmonic generation (SHG), third harmonic generation (THG), fluorescence lifetime (FLIM), and coherent anti-Stokes Raman Scattering (CARS) with relevance to clinical applications in ophthalmology. The different imaging modalities are discussed highlighting the particular strength that each has for functional tissue imaging. MPM is compared with current clinical ophthalmological imaging techniques such as reflectance confocal microscopy, optical coherence tomography, and fluorescence imaging. In addition, we discuss the future prospects for MPM in disease detection and clinical monitoring of disease progression, understanding fundamental disease mechanisms, and real-time monitoring of drug delivery.
我们回顾了多光子显微镜（MPM），包括双光子自发荧光（2PAF）、二次谐波产生（SHG）、三次谐波产生（THG）、荧光寿命成像（FLIM）以及相干反斯托克斯拉曼散射（CARS），并探讨了其在眼科临床应用中的相关性。讨论了不同的成像方式，突出了每种方式在功能性组织成像方面的独特优势。将MPM与当前临床眼科成像技术如反射式共聚焦显微镜、光学相干断层扫描和荧光成像进行了比较。此外，我们还讨论了MPM在疾病检测、疾病进展的临床监测、理解基本疾病机制以及药物递送实时监测方面的未来前景。
